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A giant chlorophyll–protein complex induced by iron deficiency in cyanobacteria
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Cyanobacteria are abundant throughout most of the world's water bodies and contribute significantly to global primary productivity through oxygenic photosynthesis. This reaction is catalysed by two membrane-bound protein complexes, photosystem I (PSI) and photosystem II (PSII), which both contain chlorophyll-binding subunits functioning as an internal antenna. In addition, phycobilisomes act as peripheral antenna systems, but no additional light-harvesting systems have been found under normal growth conditions. Iron deficiency, which is often the limiting factor for cyanobacterial growth in aquatic ecosystems, leads to the induction of additional proteins such as IsiA (ref. 3). Although IsiA has been implicated in chlorophyll storage, energy absorption and protection against excessive light, its precise molecular function and association to other proteins is unknown. Here we report the purification of a specific PSI-IsiA supercomplex, which is abundant under conditions of iron limitation. Electron microscopy shows that this supercomplex consists of trimeric PSI surrounded by a closed ring of 18 IsiA proteins binding around 180 chlorophyll molecules. We provide a structural characterization of an additional chlorophyll-containing, membrane-integral antenna in a cyanobacterial photosystem.
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“A striking functional homologue of the higher plant antenna was recently discovered in cyanobacteria (58) that surprisingly consists of completely different light harvesting proteins than those in plastids and that was hence reinvented—not inherited—during plastid evolution.”
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References 25 · Cited by 355
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Metadata
- Authors
- Boekema, E. J., Hifney, A., Yakushevska, A. E., Piotrowski, M., Keegstra, W., Berry, S., Michel, K.-P., Pistorius, E. K., & Kruip, J.
- Affiliations
- University of Groningen (NL)
- Bielefeld University (DE)
- Ruhr University Bochum (DE)
- Corresponding author
- Pistorius, E. K.
- Kruip, J.
- Venue
- Nature
- Published
- 2001-08
- Volume
- 412
- Issue
- 6848
- Pages
- 745-748
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2038498610
- Cited by
- 355
- References
- 25
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 11 · 2023: 9 · 2022: 5 · 2021: 9 · 2020: 13 · 2019: 7 · 2018: 8 · 2017: 9 · 2016: 10 · 2015: 13 · 2014: 18 · 2013: 5 · 2012: 9
- FWCI
- 9.36
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- other-oa
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion, other-oa)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Metalloenzymes and iron-sulfur proteins
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
- Keywords
- cyanobacteria, iron deficiency, photosystem I, IsiA, chlorophyll-protein complexes, supercomplexes, light-harvesting antenna, photosynthesis
- MeSH terms
- Cyanobacteria, Bacterial Proteins, Bacteriochlorophylls, Carrier Proteins, Iron, Protein Binding, Photosynthetic Reaction Center Complex Proteins, Light-Harvesting Protein Complexes, Phycobilisomes, Macromolecular Substances
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