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Energetic optimization of ion conduction rate by the K+ selectivity filter

Morais-Cabral, João H., Zhou, Yufeng, MacKinnon, Roderick·2001·Nature
DOI 10.1038/35102000
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Abstract

The K+ selectivity filter catalyses the dehydration, transfer and rehydration of a K+ ion in about ten nanoseconds. This physical process is central to the production of electrical signals in biology. Here we show how nearly diffusion-limited rates are achieved, by analysing ion conduction and the corresponding crystallographic ion distribution in the selectivity filter of the KcsA K+ channel. Measurements with K+ and its slightly larger analogue, Rb+, lead us to conclude that the selectivity filter usually contains two K+ ions separated by one water molecule. The two ions move in a concerted fashion between two configurations, K+-water-K+-water (1,3 configuration) and water-K+-water-K+ (2,4 configuration), until a third ion enters, displacing the ion on the opposite side of the queue. For K+, the energy difference between the 1,3 and 2,4 configurations is close to zero, the condition of maximum conduction rate. The energetic balance between these configurations is a clear example of evolutionary optimization of protein function.

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Crossref · 10.1038/35102000

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  • The channel might display multi-ion conduction phenomena akin to those long-known in K+ channels.

    “The channel might therefore display multi-ion conduction phenomena akin to those long-known in K+ channels25,26.”

    From Crystal structures of a double-barrelled fluoride ion channel (2015) · cites this paper as [26]

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Research works printed but not linked: 27

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Metadata

Authors
Morais-Cabral, J. H., Zhou, Y., & MacKinnon, R.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2001-11
Volume
414
Issue
6859
Pages
37-42
ISSN
  • 0028-0836
  • 1476-4687
DOI
10.1038/35102000
Cited by
761
References
0
Language
en
Licence
www.springer.com/tdm (tdm)

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