The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Tandem binding in crystals of a trp represser/operator half-site complex
Abstract
The crystal structure of trp repressor tandemly bound in a 2:1 complex to a 16-base-pair palindromic DNA containing a central trp operator half-site has been determined and refined to 2.4 A resolution. Despite dramatically different DNA sequence contexts and crystallization conditions, the protein/DNA interface is essentially identical to that seen in the original trp repressor/operator complex structure. Water-mediated sequence recognition by trp repressor is likely to be related to the unusual end-on approach of the recognition helix (E), which allows sharing of the major groove by tandem dimers. The tandem complex model accounts for the mutational sensitivity of all trp operator base pairs. The structure also provides the first detailed view of the tandem interaction, revealing a key role for the amino-terminal arms.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The two Fur dimers are positioned on almost opposite sides of the double helix, in contrast to the positioning of other prokaryotic helix-turn-helix motif class repressors, such as the trp repressor 41 .”
From Mechanistic insights into metal ion activation and operator recognition by the ferric uptake regulator (2015) · cites this paper as [41]
Citations
Cites
MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures
Kraulis, P. J. (1991) · Journal of Applied Crystallography · doi:10.1107/s0021889891004399 · Cited by 14,011
The protein data bank: A computer-based archival file for macromolecular structures
Bernstein, F. C., Koetzle, T. F., Williams, G. J. B., Meyer, E. F., Brice, M. D., Rodgers, J. R., Kennard, O., Shimanouchi, T., & Tasumi, M. (1977) · Journal of Molecular Biology · doi:10.1016/s0022-2836(77)80200-3 · Cited by 8,574
Principles of Nucleic Acid Structure
Neidle, S., & Sanderson, M. (2008) · Elsevier eBooks · doi:10.1016/b978-0-12-369507-9.x5001-8 · Cited by 4,519
Principles of Nucleic Acid Structure
Saenger, W. (1984) · Springer advanced texts in chemistry · doi:10.1007/978-1-4612-5190-3 · Cited by 4,271
Free R value: a novel statistical quantity for assessing the accuracy of crystal structures
Brünger, A. T. (1992) · Nature · doi:10.1038/355472a0 · Cited by 3,877
Crystallographic R Factor Refinement by Molecular Dynamics
Brünger, A. T., Kuriyan, J., & Karplus, M. (1987) · Science · doi:10.1126/science.235.4787.458 · Cited by 1,950
Improved Fourier coefficients for maps using phases from partial structures with errors
Read, R. J. (1986) · Acta Crystallographica Section A Foundations of Crystallography · doi:10.1107/s0108767386099622 · Cited by 1,870
Crystal structure of trp represser/operator complex at atomic resolution
Otwinowski, Z., Schevitz, R. W., Zhang, R.-G., Lawson, C. L., Joachimiak, A., Marmorstein, R. Q., Luisi, B. F., & Sigler, P. B. (1988) · Nature · doi:10.1038/335321a0 · Cited by 908
Self-citationHelntz, N. (1991) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(91)90161-n · Cited by 892
Nucleic Acids and Molecular Biology
Eckstein, F., & Lilley, D. M. J. (1994) · Nucleic acids and molecular biology · doi:10.1007/978-3-642-78666-2 · Cited by 769
Research works printed but not linked: 3
- Luisi, B. F. & Sigler, P. B. Biochem. biophys. Acta 1048, 113–126 (1990).
- Roussel, A. & Cambillau, C. in Silicon Graphics Geometry Partner Directory 77–78 (Silicon Graphics, 1989).
- Hodel, A., Kim, S.-H. & Brünger, A. T. Acta crystallogr. A. (in the press).
Cited by
Sorted by citations
Structure of the metal-ion-activated diphtheria toxin repressor/ tox operator complex
White, A., Ding, X., vanderSpek, J. C., Murphy, J. R., & Ringe, D. (1998) · Nature · doi:10.1038/28893 · Cited by 180
Deng, Z., Wang, Q., Liu, Z., Zhang, M., Machado, A. C. D., Chiu, T.-P., Feng, C., Zhang, Q., Yu, L., Qi, L., et al. (2015) · Nature Communications · doi:10.1038/ncomms8642 · Cited by 117
Metadata
- Authors
- Lawson, C. L., & Carey, J.
- Affiliations
- Brookhaven National Laboratory (US)
- Princeton University (US)
- Venue
- Nature
- Published
- 1993-11
- Volume
- 366
- Issue
- 6451
- Pages
- 178-182
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1997329250
- Cited by
- 145
- References
- 31
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2020: 2 · 2018: 1 · 2017: 1 · 2015: 3 · 2014: 1 · 2012: 1
- FWCI
- 18.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- trp repressor, protein-DNA interface, palindromic DNA, recognition helix, major groove, transcriptional repression, DNA sequence recognition
- MeSH terms
- Bacterial Proteins, Base Sequence, Computer Graphics, DNA, Bacterial, Escherichia coli, Models, Molecular, Molecular Sequence Data, Operator Regions, Genetic, Protein Binding, Repressor Proteins, Crystallography, X-Ray, Escherichia coli Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Tandem binding in crystals of a trp represser/operator half-site complex. https://app.cochat.ai/papers/10.1038/366178a0
- The paper
- Lawson, C. L., & Carey, J. (1993). Tandem binding in crystals of a trp represser/operator half-site complex. Nature. https://doi.org/10.1038/366178a0
