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A BDNF autocrine loop in adult sensory neurons prevents cell death
Abstract
During the initial phase of their development, sensory neurons of the dorsal root ganglion (DRG) require target-derived trophic support for their survival, but as they mature they lose this requirement. Because many of these neurons express BDNF (brain-derived neurotrophic factor) messenger RNA, we hypothesized that BDNF might act as an autocrine survival factor in adult DRG neurons, thus explaining their lack of dependence on exogenous growth factors. When cultured adult DRG cells were treated with antisense oligonucleotides to BDNF, expression of BDNF protein was reduced by 80%, and neuronal survival was reduced by 35%. These neurons could be rescued by exogenous BDNF or neurotrophin-3, but not by other growth factors. Similar results were obtained with single-neuron microcultures, whereas microcultures derived from mutant mice lacking BDNF were unaffected by antisense oligonucleotides. Our results strongly support an autocrine role for BDNF in mediating the survival of a subpopulation of adult DRG neurons.
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“Through an autocrine loop initiated by BDNF, these newly generated neurons are also better protected against cell death (Acheson et al., 1995).”
From Exercise-Mediated Neurogenesis in the Hippocampus via BDNF (2018) · cites this paper as Acheson et al., 1995
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Metadata
- Authors
- Acheson, A., Conover, J. C., Fandl, J. P., DeChiara, T. M., Russell, M., Thadani, A., Squinto, S. P., Yancopoulos, G. D., & Lindsay, R. M.
- Affiliations
- Regeneron (United States) (US)
- Venue
- Nature
- Published
- 1995-03
- Volume
- 374
- Issue
- 6521
- Pages
- 450-453
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2000304313
- Cited by
- 765
- References
- 32
- Citations by year
- 2026: 12 · 2025: 19 · 2024: 21 · 2023: 23 · 2022: 30 · 2021: 33 · 2020: 25 · 2019: 28 · 2018: 42 · 2017: 35 · 2016: 27 · 2015: 28 · 2014: 27 · 2013: 26 · 2012: 17
- FWCI
- 16.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Health Sciences › Medicine › Physiology (medical) › Pain Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Hereditary Neurological Disorders
- Keywords
- brain-derived neurotrophic factor, autocrine signaling, dorsal root ganglion neurons, sensory neuron survival, neuronal cell death, neurotrophin-3, antisense oligonucleotides, growth factor dependence
- MeSH terms
- Aging, Animals, Cells, Cultured, Ganglia, Spinal, Nerve Tissue Proteins, Neurons, Afferent, Polysaccharides, RNA, Messenger, Oligonucleotides, Antisense, Cell Death, Brain-Derived Neurotrophic Factor, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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