The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathway
Abstract
The 20S cyclosome complex (also known as the anaphase-promoting complex) has ubiquitin ligase activity and is required for mitotic cyclin destruction and sister chromatid separation. The formation and activation of the 20S cyclosome complex is regulated by an unknown mechanism. Here we show that Cut4 (ref. 6) is an essential component of the cyclosome in fission yeast. Cut4 shares sequence similarity with BimE, a protein that regulates mitosis in Aspergillus nidulans. Mutations in cut4 result in hypersensitivity to cyclic AMP and to stress-inducing heavy metals, inhibition of the onset of anaphase, disruption of the 20S complex, and inhibition of mitotic cyclin ubiquitination. These phenotypes are fully suppressed by cAMP phosphodiesterase and the protein kinase A (PKA) regulatory subunit and weakly suppressed by Sti1 (an activator of the Hsp70 and Hsp90 chaperones). Suppression correlates with the amount of 20S complex, indicating that cyclosome formation and activation is inhibited by the cAMP/PKA pathway.
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“A similar relationship exists in S. pombe (Yamashita et al., 1996).”
From Cdc28 provides a molecular link between Hsp90, morphogenesis, and cell cycle progression in Candida albicans (2012) · cites this paper as Yamashita et al., 1996
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Cites
Antibodies, a laboratory manual
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[12] One-step gene disruption in yeast
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Funabiki, H., Hagan, I., Uzawa, S., & Yanagida, M. (1993) · The Journal of Cell Biology · doi:10.1083/jcb.121.5.961 · Cited by 516
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Cut2 proteolysis required for sister-chromatid separation in fission yeast
Funabiki, H., Yamano, H., Kumada, K., Nagao, K., Hunt, T., & Yanagida, M. (1996) · Nature · doi:10.1038/381438a0 · Cited by 453
Self-citationTugendreich, S., Tomkiel, J. E., Earnshaw, W. C., & Hieter, P. (1995) · Cell · doi:10.1016/0092-8674(95)90336-4 · Cited by 364
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- Ishii, T., Kumada, K., Toda, T. & Yanagida, M. EMBO J. (in the press).
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Hershko, A., & Ciechanover, A. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.425 · Cited by 8,840
The Ubiquitin-Proteasome Proteolytic Pathway: Destruction for the Sake of Construction
Glickman, M. H., & Ciechanover, A. J. (2002) · Physiological Reviews · doi:10.1152/physrev.00027.2001 · Cited by 4,296
The 90-kDa Molecular Chaperone Family
Csermely, P., Schnaider, T., Sőti, C., Prohászka, Z., & Nardai, G. (1998) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(98)00013-8 · Cited by 1,024
The Phosphatase Cdc14 Triggers Mitotic Exit by Reversal of Cdk-Dependent Phosphorylation
Visintin, R., Craig, K. L., Hwang, E. S., Prinz, S., Tyers, M., & Amon, A. (1998) · Molecular Cell · doi:10.1016/s1097-2765(00)80286-5 · Cited by 725
Whose end is destruction: cell division and the anaphase-promoting complexPreprint
Zachariae, W., & Nasmyth, K. (1999) · Genes & Development · doi:10.1101/gad.13.16.2039 · Cited by 667
Metadata
- Authors
- Yamashita, Y. M., Nakaseko, Y., Samejima, I., Kumada, K., Yamada, H., Michaelson, D., & Yanagida, M.
- Affiliations
- Kyoto University (JP)
- Venue
- Nature
- Published
- 1996-11
- Volume
- 384
- Issue
- 6606
- Pages
- 276-279
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1990634825
- Cited by
- 163
- References
- 26
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2022: 2 · 2020: 1 · 2019: 1 · 2018: 4 · 2016: 4 · 2014: 5 · 2013: 6 · 2012: 3
- FWCI
- 8.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- anaphase-promoting complex, cAMP/PKA signaling, protein kinase A regulatory subunit, cAMP phosphodiesterase, STI1, sister chromatid separation, anaphase onset, heavy metal stress, Schizosaccharomyces pombe, ubiquitin ligase
- MeSH terms
- Cyclic AMP, Anaphase, Cloning, Molecular, Genes, Fungal, Ligases, Mutation, Schizosaccharomyces, Cyclins, Cyclic AMP-Dependent Protein Kinases, Metals, Heavy
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