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Cited by 179Barrier Structure and Function StudiesRNA and protein synthesis mechanismsNeuroscience and Neuropharmacology Research

Translation in astrocyte distal processes sets molecular heterogeneity at the gliovascular interface

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Boulay, Anne- Cécile, Saubaméa, Bruno, Adam, Nicolas, Chasseigneaux, Stéphanie, Mazaré, Noémie, Gilbert, Alice, Bahin, Mathieu, Bastianelli, Leïla +10 more·2017·Cell Discovery
DOI 10.1038/celldisc.2017.5PMC PMC5368712
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Abstract

Astrocytes send out long processes that are terminated by endfeet at the vascular surface and regulate vascular functions as well as homeostasis at the vascular interface. To date, the astroglial mechanisms underlying these functions have been poorly addressed. Here we demonstrate that a subset of messenger RNAs is distributed in astrocyte endfeet. We identified, among this transcriptome, a pool of messenger RNAs bound to ribosomes, the endfeetome, that primarily encodes for secreted and membrane proteins. We detected nascent protein synthesis in astrocyte endfeet. Finally, we determined the presence of smooth and rough endoplasmic reticulum and the Golgi apparatus in astrocyte perivascular processes and endfeet, suggesting for local maturation of membrane and secreted proteins. These results demonstrate for the first time that protein synthesis occurs in astrocyte perivascular distal processes that may sustain their structural and functional polarization at the vascular interface.

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Authors
Boulay, A. C., Saubaméa, B., Adam, N., Chasseigneaux, S., Mazaré, N., Gilbert, A., Bahin, M., Bastianelli, L., Blugeon, C., Perrin, S., Pouch, J., Ducos, B., Le Crom, S., Genovesio, A., Chrétien, F., Declèves, X., Laplanche, J.-L., & Cohen-Salmon, M.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Inserm (FR)
  • École Normale Supérieure - PSL (FR)
  • Institut Pasteur (FR)
ORCID
Venue
Cell Discovery
Publisher
Springer Science and Business Media LLC
Published
2017-03-28
Volume
3
Issue
1
Pages
17005-17005
ISSN
2056-5968 (ISSN-L)
DOI
10.1038/celldisc.2017.5
PMC
PMC5368712
OpenAlex
W2597423760
Cited by
179
References
71
Citations by year
2026: 20 · 2025: 17 · 2024: 14 · 2023: 31 · 2022: 23 · 2021: 23 · 2020: 20 · 2019: 19 · 2018: 8 · 2017: 4
FWCI
6.96
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Discovery (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • HAL (submittedVersion, cc-by)
Funders
  • Agence Nationale de la Recherche (ANR-10-INBS-0009)
  • Fondation pour la Recherche Médicale
Topics
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
astrocytes, astrocyte endfeet, mRNA localization, nascent protein synthesis, ribosome-bound mRNA, secreted proteins, membrane proteins, endoplasmic reticulum, Golgi apparatus, astrocyte polarization, astrocyte transcriptome

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