RNA polymerase II kinetics in polo polyadenylation signal selection
Open accessAbstract
Regulated alternative polyadenylation is an important feature of gene expression, but how gene transcription rate affects this process remains to be investigated. polo is a cell-cycle gene that uses two poly(A) signals in the 3' untranslated region (UTR) to produce alternative messenger RNAs that differ in their 3'UTR length. Using a mutant Drosophila strain that has a lower transcriptional elongation rate, we show that transcription kinetics can determine alternative poly(A) site selection. The physiological consequences of incorrect polo poly(A) site choice are of vital importance; transgenic flies lacking the distal poly(A) signal cannot produce the longer transcript and die at the pupa stage due to a failure in the proliferation of the precursor cells of the abdomen, the histoblasts. This is due to the low translation efficiency of the shorter transcript produced by proximal poly(A) site usage. Our results show that correct polo poly(A) site selection functions to provide the correct levels of protein expression necessary for histoblast proliferation, and that the kinetics of RNA polymerase II have an important role in the mechanism of alternative polyadenylation.
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On several genes possessing alternative poly(A) sites, a slow RNA Pol II mutant shows a switch to upstream poly(A) sites, while a fast mutant shows a slight downstream switch.
“On several genes possessing alternative poly(A) sites, a slow RNA Pol II mutant shows a switch to the use of upstream poly(A) sites (Pinto et al, 2011; Liu et al, 2017; Geisberg et al, 2020; Yague‐Sanz et al, 2020), while a fast RNA Pol II mutant shows a slight downstream switch in poly(A) site usage (Geisberg et al, 2020).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Pinto et al, 2011
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References 97 · Cited by 136
Research works printed but not linked: 1
- Sullivan W, Ashburner M, Hawley RS (eds) (2000) Drosophila Protocols. Cold Spring Harbor, New York, USA: Cold Spring Harbor Laboratory Press
- Authors
- Pinto, P. A. B., Henriques, T., Freitas, M. O., Martins, T., Domingues, R. G., Wyrzykowska, P. S., Coelho, P. A., Carmo, A. M., Sunkel, C. E., Proudfoot, N. J., & Moreira, A.
- Affiliations
- Universidade do Porto (PT)
- University of Oxford (GB)
- Corresponding author
- Moreira, Alexandra
- ORCID
- Venue
- The EMBO Journal
- Published
- 2011-05-20
- Volume
- 30
- Issue
- 12
- Pages
- 2431-2444
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC3116286
- OpenAlex
- W2149146886
- Cited by
- 136
- References
- 97
- Citations by year
- 2025: 4 · 2024: 7 · 2023: 9 · 2022: 6 · 2021: 10 · 2020: 11 · 2019: 7 · 2018: 10 · 2017: 12 · 2016: 9 · 2015: 12 · 2014: 13 · 2013: 12 · 2012: 10
- FWCI
- 5.03
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Wellcome Trust
- Headcase Cancer Trust
- Institut National de la Santé et de la Recherche Médicale
- Fundação para a Ciência e a Tecnologia
- European Regional Development Fund
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- alternative polyadenylation, poly(A) site selection, RNA polymerase II, 3' untranslated region, translation efficiency, Drosophila, cell cycle genes, mRNA isoforms
- MeSH terms
- Animals, Cell Survival, Drosophila melanogaster, Kinetics, Poly A, RNA Polymerase II, Genetic Variation, Signal Transduction, Protein Serine-Threonine Kinases, 3' Untranslated Regions, Polyadenylation, Drosophila Proteins, Animals, Genetically Modified, Cell Proliferation
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