The RhoA-associated protein Citron-N controls dendritic spine maintenance by interacting with spine-associated Golgi compartments
Open accessAbstract
Dendritic spines are highly dynamic protuberances that are thought to be crucial for learning and memory. Although it is well known that actin filaments and membrane dynamics regulate spine plasticity, how these two events are linked locally is less clear. Here, we provide evidence that Citron-N (CIT-N), a binding partner of the small GTPase RhoA, is associated with the actin filaments and Golgi compartments of dendritic spines. We also show that CIT-N is required for recruiting F-actin and Golgi membranes at spines of in vitro-grown neurons. Studies in knockout mice show that this protein is essential for the maturation of dendritic spines. We suggest that CIT-N might function as a scaffold protein in spine organization through its ability to bind to Golgi membranes and by affecting actin remodelling.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Citron N maintains Golgi architecture by modulating local actin polymerization together with ROCK.
“Citron N is enriched in dendritic spines and is associated with the endomembrane system, in particular the Golgi complex, where it maintains Golgi architecture by modulating local actin polymerization together with ROCK [126,127].”
From RhoA Signaling in Neurodegenerative Diseases (2022) · cites this paper as [127]
Citron N is enriched in dendritic spines and is associated with the endomembrane system, in particular the Golgi complex.
“Citron N is enriched in dendritic spines and is associated with the endomembrane system, in particular the Golgi complex, where it maintains Golgi architecture by modulating local actin polymerization together with ROCK [126,127].”
From RhoA Signaling in Neurodegenerative Diseases (2022) · cites this paper as [127]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus
Zhang, W., Vazquez, L., Apperson, M., & Kennedy, M. B. (1999) · The Journal of Neuroscience · doi:10.1523/jneurosci.19-01-00096.1999 · Cited by 190 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- Goslin K, Banker GA (1991) Culturing Nerve Cells, pp 251-281. Cambridge, MA, USA: MIT Press
Cited by
Sorted by citations
RhoA Signaling in Neurodegenerative Diseases
Schmidt, S. I., Blaabjerg, M., Freude, K., & Meyer, M. (2022) · Cells · doi:10.3390/cells11091520 · Cited by 110 · see citing works
Dendritic remodeling of D1 neurons by RhoA/Rho-kinase mediates depression-like behavior
Fox, M. E., Chandra, R., Menken, M. S., Larkin, E. J., Nam, H., Engeln, M., Francis, T. C., & Lobo, M. K. (2020) · Molecular Psychiatry · doi:10.1038/s41380-018-0211-5 · Cited by 100 · see citing works
- Authors
- Camera, P., Schubert, V., Pellegrino, M., Berto, G., Vercelli, A., Muzzi, P., Hirsch, E., Altruda, F., Dotti, C. G., & Di Cunto, F.
- Affiliations
- University of Turin (IT)
- Ospedale San Luigi Gonzaga (IT)
- ORCID
- Venue
- The EMBO Reports
- Published
- 2008-02-29
- Volume
- 9
- Issue
- 4
- Pages
- 384-392
- ISSN
- 1469-221X (ISSN-L)
- 1469-3178
- PMC
- PMC2288759
- OpenAlex
- W2102312146
- Cited by
- 25
- References
- 1
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 1 · 2019: 2 · 2018: 3 · 2017: 1 · 2014: 3 · 2013: 2
- FWCI
- 0.55
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- EMBO Reports (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Institutional Research Information System University of Turin (University of Turin) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- RhoA, dendritic spines, F-actin, Golgi membranes, spine maturation, actin cytoskeleton remodeling, scaffold proteins, spine plasticity, learning and memory
- MeSH terms
- Actins, Animals, Cells, Cultured, Golgi Apparatus, Microscopy, Fluorescence, Neurons, Immunoblotting, Protein Serine-Threonine Kinases, Immunoprecipitation, Intracellular Signaling Peptides and Proteins, Dendritic Spines, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
