Characterization of anti-MERS-CoV antibodies against various recombinant structural antigens of MERS-CoV in an imported case in China
Open accessAbstract
The first imported case of Middle East respiratory syndrome (MERS) in China recently occurred, allowing for the characterization of antibody titers in a series of the patient's sera using the following methods based on recombinant viral structural antigens: inactivated MERS coronavirus (MERS-CoV) enzyme-linked immunosorbent assay (ELISA), recombinant MERS-CoV spike (S, or fragments of S) ELISA, nucleoprotein (NP) ELISA and MERS S pseudovirus particle-based neutralization test (ppNT). A longitudinal profile of the infection showed that seroconversion detected by ELISAs based on the recombinant extracellular domain, S, S1 and receptor-binding domain (RBD) antigens occurred as early as neutralizing antibodies were detected by the ppNT and earlier than antibodies were detected by the inactivated MERS-CoV and N-terminal domain (NTD) ELISAs. Antibodies detected by the NP ELISA occurred last. Strong correlations were found between the S1, RBD and NP ELISAs and the inactivated MERS-CoV ELISA. The S and RBD ELISAs were highly correlated with the commercial S1 ELISA. The S ELISA strongly correlated with the ppNT, although the MERS-CoV, S1, NTD and RBD ELISAs were also significantly correlated with the ppNT (P<0.001).
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Polyclonal antibodies against multiple non-RBD spike epitopes might block viral attachment, add neutralizing activity, or prevent post-attachment fusion.
“A polyclonal antibody against multiple epitopes of the S protein beyond the RBD might, for example, inhibit viral attachment156–160, provide additional neutralizing activity161 and/or prevent post-attachment fusion162.”
From Immunological mechanisms of vaccine-induced protection against COVID-19 in humans (2021) · cites this paper as [161]
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- Authors
- Wang, W., Wang, H., Deng, Y., Song, T., Lan, J., Wu, G., Ke, C., & Tan, W.
- Affiliations
- Chinese Center For Disease Control and Prevention (CN)
- Guangdong Provincial Center for Disease Control and Prevention (CN)
- Corresponding author
- Tan, Wenjie
- Venue
- Emerging Microbes & Infections
- Publisher
- Informa UK Limited
- Published
- 2016-01
- Volume
- 5
- Issue
- 1
- Pages
- 1-12
- ISSN
- 2222-1751 (ISSN-L)
- PMC
- PMC5148018
- OpenAlex
- W2549271666
- Cited by
- 25
- References
- 13
- Citations by year
- 2024: 2 · 2022: 1 · 2021: 5 · 2020: 8 · 2019: 4 · 2018: 2 · 2017: 3
- FWCI
- 2.77
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Emerging Microbes & Infections (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Chinese Academy of Sciences
- National Key Research and Development Program of China (2016YFD0500301, 2011CB504704)
- Institute of Microbiology, Chinese Academy of Sciences
- Topics
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Outbreaks Research
- Keywords
- MERS-CoV, MERS, imported infections, seroconversion, neutralizing antibodies, spike protein, receptor binding domain, nucleocapsid protein, pseudovirus neutralization, antibody titer correlation, N-terminal domain
- MeSH terms
- Antibodies, Viral, Antigens, Viral, China, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin G, Immunoglobulin M, Male, Neutralization Tests, Nucleoproteins, Recombinant Proteins, Coronavirus Infections, Antibodies, Neutralizing, Spike Glycoprotein, Coronavirus, Middle East Respiratory Syndrome Coronavirus
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