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Cited by 109Birth, Development, and HealthAdipokines, Inflammation, and Metabolic DiseasesAdipose Tissue and Metabolism

Catch-up growth following intra-uterine growth-restriction programmes an insulin-resistant phenotype in adipose tissue

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Berends, L M, Fernandez-Twinn, D S, Martin-Gronert, M S, Cripps, R L, Ozanne, S E·2013·International Journal of Obesity
DOI 10.1038/ijo.2012.196PMC PMC3734734
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Abstract

BACKGROUND: It is now widely accepted that the early-life nutritional environment is important in determining susceptibility to metabolic diseases. In particular, intra-uterine growth restriction followed by accelerated postnatal growth is associated with an increased risk of obesity, type-2 diabetes and other features of the metabolic syndrome. The mechanisms underlying these observations are not fully understood. AIM: Using a well-established maternal protein-restriction rodent model, our aim was to determine if exposure to mismatched nutrition in early-life programmes adipose tissue structure and function, and expression of key components of the insulin-signalling pathway. METHODS: Offspring of dams fed a low-protein (8%) diet during pregnancy were suckled by control (20%)-fed dams to drive catch-up growth. This 'recuperated' group was compared with offspring of dams fed a 20% protein diet during pregnancy and lactation (control group). Epididymal adipose tissue from 22-day and 3-month-old control and recuperated male rats was studied using histological analysis. Expression and phosphorylation of insulin-signalling proteins and gene expression were assessed by western blotting and reverse-transcriptase PCR, respectively. RESULTS: Recuperated offspring at both ages had larger adipocytes (P<0.001). Fasting serum glucose, insulin and leptin levels were comparable between groups but increased with age. Recuperated offspring had reduced expression of IRS-1 (P<0.01) and PI3K p110β (P<0.001) in adipose tissue. In adult recuperated rats, Akt phosphorylation (P<0.01) and protein levels of Akt-2 (P<0.01) were also reduced. Messenger RNA expression levels of these proteins were not different, indicating a post-transcriptional effect. CONCLUSION: Early-life nutrition programmes alterations in adipocyte cell size and impairs the protein expression of several insulin-signalling proteins through post-transcriptional mechanisms. These indices may represent early markers of insulin resistance and metabolic disease risk.

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  • The effect of a maternal low protein diet on offspring adiposity is still observed even when the offspring is cross-fostered.

    “Studies in rats have shown that the effect of a maternal low protein diet on offspring adiposity is still observed even when the offspring is cross-fostered [80].”

    From Developmental programming of offspring adipose tissue biology and obesity risk (2021) · cites this paper as [80]

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Authors
Berends, L. M., Fernandez-Twinn, D. S., Martin-Gronert, M. S., Cripps, R. L., & Ozanne, S. E.
Affiliations
University of Cambridge (GB)
Corresponding author
Berends, L M
ORCID
Ozanne, S E
Venue
International Journal of Obesity
Publisher
Springer Science and Business Media LLC
Published
2012-12-11
Volume
37
Issue
8
Pages
1051-1057
ISSN
  • 0307-0565 (ISSN-L)
  • 1476-5497
DOI
10.1038/ijo.2012.196
PMC
PMC3734734
OpenAlex
W2036734614
Cited by
109
References
52
Citations by year
2026: 3 · 2025: 2 · 2024: 1 · 2023: 6 · 2022: 7 · 2021: 7 · 2020: 7 · 2019: 14 · 2018: 11 · 2017: 14 · 2016: 5 · 2015: 15 · 2014: 9 · 2013: 8
FWCI
9.00
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Obesity (publishedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • UEA Digital Repository (University of East Anglia) (submittedVersion)
  • Apollo (University of Cambridge) (submittedVersion, cc-by-nc-nd)
  • Apollo
Funders
  • Wellcome Trust
  • British Heart Foundation (PG/09/037/27387, FS/09/029/27902)
  • University of Cambridge
  • Cambridge University Hospitals
  • Directorate for Biological Sciences
  • Medical Research Council (MC_PC_12012)
  • Biotechnology and Biological Sciences Research Council (BB/F015364/1, BB/E00797X/1)
Topics
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
catch-up growth, fetal growth restriction, maternal protein restriction, adipose tissue, insulin resistance, insulin signaling, IRS-1, PI3K p110β, AKT2, adipocyte size, metabolic syndrome, early life nutrition, post-transcriptional regulation, type 2 diabetes risk, obesity risk
MeSH terms
Adipose Tissue, Animals, Body Weight, Diabetes Mellitus, Type 2, Female, Fetal Growth Retardation, Insulin, Insulin Resistance, Male, Obesity, Phenotype, Phosphorylation, Pregnancy, Prenatal Exposure Delayed Effects, Blotting, Western, Gene Expression, Rats, Wistar, Adipocytes, Metabolic Syndrome, Fetal Development, Proto-Oncogene Proteins c-akt, Rats

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