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EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols
Open accessAbstract
The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry with fully standardized laboratory procedures and antibody panels in order to achieve maximally comparable results among different laboratories. This required the selection of optimal combinations of compatible fluorochromes and the design and evaluation of adequate standard operating procedures (SOPs) for instrument setup, fluorescence compensation and sample preparation. Additionally, we developed software tools for the evaluation of individual antibody reagents and antibody panels. Each section describes what has been evaluated experimentally versus adopted based on existing data and experience. Multicentric evaluation demonstrated high levels of reproducibility based on strict implementation of the EuroFlow SOPs and antibody panels. Overall, the 6 years of extensive collaborative experiments and the analysis of hundreds of cell samples of patients and healthy controls in the EuroFlow centers have provided for the first time laboratory protocols and software tools for fully standardized 8-color flow cytometric immunophenotyping of normal and malignant leukocytes in bone marrow and blood; this has yielded highly comparable data sets, which can be integrated in a single database.
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Nearest neighbor pattern classification
Cover, T., & Hart, P. (1967) · IEEE Transactions on Information Theory · doi:10.1109/tit.1967.1053964 · Cited by 16,936
Seventeen-colour flow cytometry: unravelling the immune system
Perfetto, S. P., Chattopadhyay, P. K., & Roederer, M. (2004) · Nature Reviews Immunology · doi:10.1038/nri1416 · Cited by 1,019
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Metadata
- Authors
- on behalf of the EuroFlow Consortium (EU-FP6, L.-C.-2.-0., Kalina, T., Flores-Montero, J., van der Velden, V. H. J., Martin-Ayuso, M., Böttcher, S., Ritgen, M., Almeida, J., Lhermitte, L., Asnafi, V, Mendonça, A., de Tute, R., Cullen, M., Sedek, L., Vidriales, M. B., Pérez, J. J., te Marvelde, J. G., Mejstrikova, E., Hrusak, O., Szczepański, T., van Dongen, J. J. M., & Orfao, A.
- Venue
- Leukemia
- Published
- 2012-09
- Volume
- 26
- Issue
- 9
- Pages
- 1986-2010
- ISSN
- 0887-6924 (ISSN-L)
- 1476-5551
- OpenAlex
- W2087296000
- Cited by
- 783
- References
- 2
- FWCI
- 20.57
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- The paper
- on behalf of the EuroFlow Consortium (EU-FP6, L.-C.-2.-0., Kalina, T., Flores-Montero, J., van der Velden, V. H. J., Martin-Ayuso, M., Böttcher, S., Ritgen, M., Almeida, J., Lhermitte, L., Asnafi, V, Mendonça, A., de Tute, R., Cullen, M., Sedek, L., Vidriales, M. B., Pérez, J. J., te Marvelde, J. G., Mejstrikova, E., Hrusak, O., . . . Orfao, A. (2012). EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. https://doi.org/10.1038/leu.2012.122
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
