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Cited by 81Cellular Mechanics and InteractionsCancer Cells and MetastasisMechanisms of cancer metastasis

Involvement of Arp2/3 complex in the process of colorectal carcinogenesis

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Otsubo, Toshiya, Iwaya, Keiichi, Mukai, Yasuo, Mizokami, Yuji, Serizawa, Hiromi, Matsuoka, Takeshi, Mukai, Kiyoshi·2004·Modern Pathology
DOI 10.1038/modpathol.3800062
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Abstract

Increased motility is one of the characteristics of cancer cells, and actin polymerization and disassembly are essential for cellular motility. Since actin-related protein (Arp) 2/3 complex acts as a nucleus for actin polymerization, in this study, we immunohistochemically investigated the expression of Arp2 and Arp3 in 175 colorectal tumors in various stages of neoplastic progression. Arp2 and Arp3 showed identical expression patterns, and both were expressed in the stromal cells around neoplastic tubules or glands and in the tumor cells themselves. The frequency of expression of Arp2 and Arp3 (Arp2 and 3) by the stromal cells increased with the atypia of the colorectal neoplasms, from 5.5% (3/55) in adenoma with mild or moderate atypia, to 11.8% (2/17) in adenoma with severe atypia, 53.3% (16/30) in intramucosal carcinoma, and 91.8% (67/73) in invasive carcinoma (P<0.0001). The frequency of expression of Arp2 and 3 in the tumor cells was similar and was 1.8% (1/55) in adenoma with mild or moderate atypia, 23.5% (4/17) in adenoma with severe atypia, 23.5% (7/30) in intramucosal carcinoma, and 32.9% (24/73) in invasive carcinoma. Expression of Arp2 and 3 by the stromal cells was significantly correlated with nuclear accumulation of p53 in the tumor cells and stromal expression of CD10. These results suggest that formation of Arp2/3 complex by both neoplastic and stromal cells contributes to the increased motility of both cell types and thus provides suitable conditions for invasion.

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  • Overexpression of the ARP2/3 complex is observed in a variety of human cancers.

    “Overexpression of the ARP2/3 complex has been observed in a variety of human cancers”

    From SHARPIN S146 phosphorylation mediates ARP2/3 interaction, cancer cell invasion and metastasis (2022) · cites this paper as Otsubo, Iwaya et al., 2004

    The Distribution of Arp2 and Arp3 section reports expression in colorectal adenomas and carcinomas, but not in a variety of cancer types. The Discussion likewise limits the study’s findings to colorectal neoplasms, so the broader claim is unsupported by this source. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Cited by

Sorted by citations

Authors
Otsubo, T., Iwaya, K., Mukai, Y., Mizokami, Y., Serizawa, H., Matsuoka, T., & Mukai, K.
Affiliations
  • Tokyo Medical University (JP)
  • Tokyo Medical University Hospital (JP)
Corresponding author
Mukai, Kiyoshi
ORCID
Venue
Modern Pathology
Publisher
Elsevier BV
Published
2004-04
Volume
17
Issue
4
Pages
461-467
ISSN
0893-3952 (ISSN-L)
DOI
10.1038/modpathol.3800062
OpenAlex
W2073607747
Cited by
81
References
1
Citations by year
2026: 1 · 2025: 1 · 2024: 5 · 2023: 5 · 2022: 2 · 2021: 6 · 2020: 1 · 2019: 3 · 2018: 5 · 2017: 5 · 2016: 4 · 2015: 6 · 2014: 2 · 2013: 7 · 2012: 4
FWCI
1.65
Type
journal article
Language
en
Open access
Licence
Locations
  • Modern Pathology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mechanisms of cancer metastasis
Keywords
Arp2/3 complex, Arp2, Arp3, colorectal carcinogenesis, colorectal adenoma, intramucosal carcinoma, invasive carcinoma, tumor stroma, actin polymerization, cell motility, p53 nuclear accumulation, CD10, tumor invasion
MeSH terms
Actins, Adenocarcinoma, Adenoma, Cytoskeletal Proteins, Humans, Immunoenzyme Techniques, Neoplasm Invasiveness, Reagent Kits, Diagnostic, Biomarkers, Tumor, Colorectal Neoplasms, Neprilysin, Stromal Cells, Disease Progression, Actin-Related Protein 2, Actin-Related Protein 3

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