The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Crystal structure of HIV-1 Tat complexed with human P-TEFb
Open accessAbstract
Regulation of the expression of the human immunodeficiency virus (HIV) genome is accomplished in large part by controlling transcription elongation. The viral protein Tat hijacks the host cell's RNA polymerase II elongation control machinery through interaction with the positive transcription elongation factor, P-TEFb, and directs the factor to promote productive elongation of HIV mRNA. Here we describe the crystal structure of the Tat.P-TEFb complex containing HIV-1 Tat, human Cdk9 (also known as CDK9), and human cyclin T1 (also known as CCNT1). Tat adopts a structure complementary to the surface of P-TEFb and makes extensive contacts, mainly with the cyclin T1 subunit of P-TEFb, but also with the T-loop of the Cdk9 subunit. The structure provides a plausible explanation for the tolerance of Tat to sequence variations at certain sites. Importantly, Tat induces significant conformational changes in P-TEFb. This finding lays a foundation for the design of compounds that would specifically inhibit the Tat.P-TEFb complex and block HIV replication.
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“Following the Tat–TAR interaction, the host super elongation complex, which includes the cellular positive transcription elongation factor p-TEFb and polymerase-associated factor 1 (PAF-1), is recruited to further boost transcription, significantly increasing the production of viral RNA transcripts (Fig. 8A) (Peterlin and Price 2006, Tahirov et al. 2010, Gu et al. 2014).”
From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Tahirov et al. 2010
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[20] Processing of X-ray diffraction data collected in oscillation mode
Otwinowski, Z., & Minor, W. (1997) · Methods in Enzymology · doi:10.1016/s0076-6879(97)76066-x · Cited by 33,475
Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination
Brünger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse‐Kunstleve, R. W., Jiang, J., Kuszewski, J. J., Nilges, M., Pannu, N., et al. (1998) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444998003254 · Cited by 15,723
WebLogo: A Sequence Logo Generator: Figure 1Preprint
Crooks, G. E., Hon, G. C., Chandonia, J., & Brenner, S. E. (2004) · Genome Research · doi:10.1101/gr.849004 · Cited by 12,480
Controlling the elongation phase of transcription with P-TEFb
Peterlin, B. M., & Price, D. H. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.06.014 · Cited by 1,144
Self-citationCrystal structure of the p27Kip1 cyclin-dependent-kinase inibitor bound to the cyclin A–Cdk2 complex
Russo, A. A., Jeffrey, P. D., Patten, A. K., Massagué, J., & Pavletich, N. P. (1996) · Nature · doi:10.1038/382325a0 · Cited by 899
Pavletich, N. P. (1999) · Journal of Molecular Biology · doi:10.1006/jmbi.1999.2640 · Cited by 746
Transcription elongation factor P-TEFb is required for HIV-1 Tat transactivation in vitroPreprint
Zhu, Y., Peery, T., Peng, J., Ramanathan, Y., Marshall, N. F., Marshall, T. K., Amendt, B. A., Mathews, M. B., & Price, D. H. (1997) · Genes & Development · doi:10.1101/gad.11.20.2622 · Cited by 700
Self-citationControl of RNA Polymerase II Elongation Potential by a Novel Carboxyl-terminal Domain Kinase
Marshall, N. F., Peng, J., Xie, Z., & Price, D. H. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.43.27176 · Cited by 662
Self-citationGlobal and regional distribution of HIV-1 genetic subtypes and recombinants in 2004
Hemelaar, J., Gouws, E., Ghys, P. D., & Osmanov, S. (2006) · AIDS · doi:10.1097/01.aids.0000247564.73009.bc · Cited by 627
Flavopiridol Inactivates P-TEFb and Blocks Most RNA Polymerase II Transcription in Vivo
Chao, S., & Price, D. H. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m102306200 · Cited by 623
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Metadata
- Authors
- Tahirov, T. H., Babayeva, N. D., Varzavand, K., Cooper, J. J., Sedore, S. C., & Price, D. H.
- Affiliations
- University of Nebraska Medical Center (US)
- University of Iowa (US)
- Corresponding author
- Tahirov, Tahir H.
- Venue
- Nature
- Published
- 2010-06
- Volume
- 465
- Issue
- 7299
- Pages
- 747-751
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC2885016
- OpenAlex
- W2127284613
- Cited by
- 314
- References
- 47
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 13 · 2023: 7 · 2022: 13 · 2021: 10 · 2020: 16 · 2019: 15 · 2018: 25 · 2017: 27 · 2016: 27 · 2015: 24 · 2014: 23 · 2013: 30 · 2012: 28
- FWCI
- 11.16
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (P30CA036727)
- National Institute of General Medical Sciences (R01GM035500, R01GM082923, GM-35500, GM082923)
- National Institute of Allergy and Infectious Diseases (AI074392, R21AI074392, R33AI074392)
- National Center for Research Resources (RR 15301, P41RR015301)
- Basic Energy Sciences
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
- Keywords
- HIV-1 Tat protein, P-TEFb, CDK9, cyclin T1, transcription elongation, protein-protein interaction, conformational change, HIV replication, antiviral drug targets
- MeSH terms
- Adenosine Triphosphate, Amino Acid Sequence, Animals, Binding Sites, Enzyme Activation, Humans, Models, Molecular, Molecular Sequence Data, Protein Binding, Protein Conformation, HIV-1, Crystallography, X-Ray, Cyclin-Dependent Kinase 9, Positive Transcriptional Elongation Factor B, tat Gene Products, Human Immunodeficiency Virus, Cyclin T
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