The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Activity-dependent relocation of the axon initial segment fine-tunes neuronal excitability
Open accessAbstract
In neurons, the axon initial segment (AIS) is a specialized region near the start of the axon that is the site of action potential initiation. The precise location of the AIS varies across and within different neuronal types, and has been linked to cells’ information-processing capabilities; however, the factors determining AIS position in individual neurons remain unknown. Here we show that changes in electrical activity can alter the location of the AIS. In dissociated hippocampal cultures, chronic depolarization with high extracellular potassium moves multiple components of the AIS, including voltage-gated sodium channels, up to 17 μm away from the soma of excitatory neurons. This movement reverses when neurons are returned to non-depolarized conditions, and depends on the activation of T- and/or L-type voltage-gated calcium channels. The AIS also moved distally when we combined long-term LED (light-emitting diode) photostimulation with sparse neuronal expression of the light-activated cation channel channelrhodopsin-2; here, burst patterning of activity was successful where regular stimulation at the same frequency failed. Furthermore, changes in AIS position correlate with alterations in current thresholds for action potential spiking. Our results show that neurons can regulate the position of an entire subcellular structure according to their ongoing levels and patterns of electrical activity. This novel form of activity-dependent plasticity may fine-tune neuronal excitability during development.
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Cites
Millisecond-timescale, genetically targeted optical control of neural activity
Boyden, E. S., Zhang, F., Bamberg, E., Nagel, G., & Deisseroth, K. (2005) · Nature Neuroscience · doi:10.1038/nn1525 · Cited by 4,861
The NEURON Simulation Environment
Hines, M. L., & Carnevale, N. T. (1997) · Neural Computation · doi:10.1162/neco.1997.9.6.1179 · Cited by 2,658
Distinct contributions of Nav1.6 and Nav1.2 in action potential initiation and backpropagation
Hu, W., Tian, C., Li, T., Yang, M., Hou, H., & Shu, Y. (2009) · Nature Neuroscience · doi:10.1038/nn.2359 · Cited by 706
Action potential generation requires a high sodium channel density in the axon initial segment
Kole, M. H. P., Ilschner, S. U., Kampa, B. M., Williams, S. R., Ruben, P. C., & Stuart, G. J. (2008) · Nature Neuroscience · doi:10.1038/nn2040 · Cited by 703
Hebb and homeostasis in neuronal plasticity
Turrigiano, G. G., & Nelson, S. B. (2000) · Current Opinion in Neurobiology · doi:10.1016/s0959-4388(00)00091-x · Cited by 683
Zhou, D., Lambert, S., Malen, P. L., Carpenter, S., Boland, L. M., & Bennett, V. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.5.1295 · Cited by 568
Magee, J. C., & Johnston, D. (1995) · The Journal of Physiology · doi:10.1113/jphysiol.1995.sp020862 · Cited by 557
A diffusion barrier maintains distribution of membrane proteins in polarized neurons
Winckler, B., Forscher, P., & Mellman, I. (1999) · Nature · doi:10.1038/17806 · Cited by 406
Cell-type-dependent molecular composition of the axon initial segment
Lorincz, A., & Nusser, Z. (2008) · The Journal of Neuroscience · doi:10.1523/jneurosci.4833-08.2008 · Cited by 382
Correlated Bursts of Activity in the Neonatal Hippocampus in Vivo
Leinekugel, X., Khazipov, R., Cannon, R., Hirase, H., Ben-Ari, Y., & Buzsáki, G. (2002) · Science · doi:10.1126/science.1071111 · Cited by 362
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Fenno, L., Yizhar, O., & Deisseroth, K. (2011) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-061010-113817 · Cited by 1,822
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Fields, R. D. (2015) · Nature Reviews Neuroscience · doi:10.1038/nrn4023 · Cited by 794
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Hochbaum, D. R., Zhao, Y., Farhi, S. L., Klapoetke, N., Werley, C. A., Kapoor, V., Zou, P., Kralj, J. M., Maclaurin, D., Smedemark-Margulies, N., et al. (2014) · Nature Methods · doi:10.1038/nmeth.3000 · Cited by 770
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Senn, V., Wolff, S. B. E., Herry, C., Grenier, F., Ehrlich, I., Gründemann, J., Fadok, J. P., Müller, C., Letzkus, J. J., & Lüthi, A. (2014) · Neuron · doi:10.1016/j.neuron.2013.11.006 · Cited by 522
Metadata
- Authors
- Grubb, M. S., & Burrone, J.
- Venue
- Nature
- Published
- 2010-06
- Volume
- 465
- Issue
- 7301
- Pages
- 1070-1074
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1989092549
- Cited by
- 655
- References
- 31
- FWCI
- 13.93
- Type
- journal article
- Language
- en
- Open access
- green
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