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Cited by 868Plant Parasitism and ResistancePlant Molecular Biology ResearchPlant biochemistry and biosynthesis

DWARF 53 acts as a repressor of strigolactone signalling in rice

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Jiang, Liang, Liu, Xue, Xiong, Guosheng, Liu, Huihui, Chen, Fulu, Wang, Lei, Meng, Xiangbing, Liu, Guifu +12 more·2013·Nature
DOI 10.1038/nature12870PMC PMC5802366
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Abstract

Strigolactones (SLs) are a group of newly identified plant hormones that control plant shoot branching. SL signalling requires the hormone-dependent interaction of DWARF 14 (D14), a probable candidate SL receptor, with DWARF 3 (D3), an F-box component of the Skp-Cullin-F-box (SCF) E3 ubiquitin ligase complex. Here we report the characterization of a dominant SL-insensitive rice (Oryza sativa) mutant dwarf 53 (d53) and the cloning of D53, which encodes a substrate of the SCF(D3) ubiquitination complex and functions as a repressor of SL signalling. Treatments with GR24, a synthetic SL analogue, cause D53 degradation via the proteasome in a manner that requires D14 and the SCF(D3) ubiquitin ligase, whereas the dominant form of D53 is resistant to SL-mediated degradation. Moreover, D53 can interact with transcriptional co-repressors known as TOPLESS-RELATED PROTEINS. Our results suggest a model of SL signalling that involves SL-dependent degradation of the D53 repressor mediated by the D14-D3 complex.

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  • Rice D53 interacts with D3, the rice ortholog of MAX2, and d3 mutants are suppressed by knockdowns of D53.

    “Moreover, rice D53 interacts with D3, which is the rice ortholog of MAX2, and d3 mutants are suppressed by knockdowns of D53 (Jiang et al., 2013; Zhou et al., 2013).”

    From Shaping plant architecture (2015) · cites this paper as Jiang et al., 2013

    The ‘Formation of a D14–D3–D53 complex’ section reports a D53–D3 interaction in a pull-down assay and says that reducing D53 expression partially rescues the d3 mutant’s signalling defect. Our copy does not identify rice D3 as the ortholog of MAX2, so that part of the sentence is not established by this source. (LLM, conf=0.86)

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  • Three different candidate repressors for the strigolactone signaling pathway have been identified: DELLA proteins, BES1, and D53.

    “Indeed, three different candidate repressors for the strigoalactone signaling pathway have been identified: DELLA proteins (Nakamura et al., 2013), BES1 (Wang et al., 2013), and D53 in rice (Jiang et al., 2013; Zhou et al., 2013).”

    From Shaping plant architecture (2015) · cites this paper as Jiang et al., 2013

  • Dominant gain of function mutations in D53 prevent SL-mediated degradation of the protein and shut off SL signaling.

    “Dominant gain of function mutations in D53 prevent SL-mediated degradation of the protein and shut off SL signaling.”

    From Shaping plant architecture (2015) · cites this paper as Jiang et al., 2013

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Research works printed but not linked: 41

  1. [1]Cook CE Whichard LP Turner B Wall ME Egley GH Germination of witchweed (Striga lutea Lour.): isolation and properties of a potent stimulant Science 154 1189 1190 1966 17780042
  2. [2]Akiyama K Matsuzaki K Hayashi H Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi Nature 435 824 827 2005 15944706
  3. [3]Gomez-Roldan V Strigolactone inhibition of shoot branching Nature 455 189 194 2008 18690209
  4. [4]Umehara M Inhibition of shoot branching by new terpenoid plant hormones Nature 455 195 200 2008 18690207
  5. [5]Domagalska MA Leyser O Signal integration in the control of shoot branching Nature Rev Mol Cell Biol 12 211 221 2011 21427763
  6. [6]Ruyter-Spira C Al-Babili S van der Krol S Bouwmeester H The biology of strigolactones Trends Plant Sci 18 72 83 2013 23182342
  7. [9]Booker J MAX3/CCD7 is a carotenoid cleavage dioxygenase required for the synthesis of a novel plant signaling molecule Curr Biol 14 1232 1238 2004 15268852
  8. [10]Booker J MAX1 encodes a cytochrome P450 family member that acts downstream of MAX3/4 to produce a carotenoid-derived branch-inhibiting hormone Dev Cell 8 443 449 2005 15737939
  9. [11]Stirnberg P Furner IJ Leyser O MAX2 participates in an SCF complex which acts locally at the node to suppress shoot branching Plant J 50 80 94 2007 17346265
  10. [13]Waters MT Specialisation within the DWARF14 protein family confers distinct responses to karrikins and strigolactones in Arabidopsis Development 139 1285 1295 2012 22357928
  11. [14]Ishikawa S Suppression of tiller bud activity in tillering dwarf mutants of rice Plant Cell Physiol 46 79 86 2005 15659436
  12. [15]Zou J The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds Plant J 48 687 698 2006 17092317
  13. [16]Arite T DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice Plant J 51 1019 1029 2007 17655651
  14. [17]Lin H DWARF27, an iron-containing protein required for the biosynthesis of strigolactones, regulates rice tiller bud outgrowth Plant Cell 21 1512 1525 2009 19470589 PMC2700539
  15. [18]Arite T d14, a strigolactone-insensitive mutant of rice, shows an accelerated outgrowth of tillers Plant Cell Physiol 50 1416 1424 2009 19542179
  16. [19]Gao Z Dwarf 88, a novel putative esterase gene affecting architecture of rice plant Plant Mol Biol 71 265 276 2009 19603144
  17. [20]Liu W Identification and characterization of HTD2: a novel gene negatively regulating tiller bud outgrowth in rice Planta 230 649 658 2009 19579033
  18. [21]Johnson X Branching genes are conserved across species. Genes controlling a novel signal in pea are coregulated by other long-distance signals Plant Physiol 142 1014 1026 2006 16980559 PMC1630745
  19. [22]Snowden KC The Decreased apical dominance1/Petunia hybrida CAROTENOID CLEAVAGE DIOXYGENASE8 gene affects branch production and plays a role in leaf senescence, root growth, and flower development Plant Cell 17 746 759 2005 15705953 PMC1069696
  20. [23]Simons JL Napoli CA Janssen BJ Plummer KM Snowden KC Analysis of the DECREASED APICAL DOMINANCE genes of petunia in the control of axillary branching Plant Physiol 143 697 706 2007 17158589 PMC1803742
  21. [24]Hamiaux C DAD2 is an α/β hydrolase likely to be involved in the perception of the plant branching hormone, strigolactone Curr Biol 22 2032 2036 2012 22959345
  22. [25]Wang Y Li J Molecular basis of plant architecture Annu Rev Plant Biol 59 253 279 2008 18444901
  23. [26]Alder A The path from β-carotene to carlactone, a strigolactone-like plant hormone Science 335 1348 1351 2012 22422982
  24. [27]Zhao LH Crystal structures of two phytohormone signal-transducing α/β hydrolases: karrikin-signaling KAI2 and strigolactone-signaling DWARF14 Cell Res 23 436 439 2013 23381136 PMC3587710
  25. [28]Kagiyama M Structures of D14 and D14L in the strigolactone and karrikin signaling pathways Genes Cells 18 147 160 2013 23301669
  26. [29]Smith SM Waters MT Strigolactones: destruction-dependent perception? Curr Biol 22 R924 R927 2012 23137691
  27. [32]Stanga JP Smith SM Briggs WR Nelson DC SUPPRESSOR OF MORE AXILLARY GROWTH2 1 controls seed germination and seedling development in Arabidopsis Plant Physiol 163 318 330 2013 23893171 PMC3762653
  28. [33]Szemenyei H Hannon M Long JA TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis Science 319 1384 1386 2008 18258861
  29. [34]Pauwels L NINJA connects the co-repressor TOPLESS to jasmonate signalling Nature 464 788 791 2010 20360743 PMC2849182
  30. [35]Fukui K New branching inhibitors and their potential as strigolactone mimics in rice Bioorg Med Chem Lett 21 4905 4908 2011 21741836
  31. [36]Fukui K Ito S Asami T Selective mimics of strigolactone actions and their potential use for controlling damage caused by root parasitic weeds Mol Plant 6 88 99 2013 23204501
  32. [37]Nelson DC F-box protein MAX2 has dual roles in karrikin and strigolactone signaling in Arabidopsis thaliana Proc Natl Acad Sci USA 108 8897 8902 2011 21555559 PMC3102411
  33. [38]Causier B Ashworth M Guo W Davies B The TOPLESS interactome: a framework for gene repression in Arabidopsis Plant Physiol 158 423 438 2012 22065421 PMC3252085
  34. [39]Long JA Ohno C Smith ZR Meyerowitz EM TOPLESS regulates apical embryonic fate in Arabidopsis Science 312 1520 1523 2006 16763149
  35. [40]Yoshida A Ohmori Y Kitano H Taguchi-Shiobara F Hirano HY Aberrant spikelet and panicle1, encoding a TOPLESS-related transcriptional co-repressor, is involved in the regulation of meristem fate in rice Plant J 70 327 339 2012 22136599
  36. [42]Gao X OsLIS-L1 encoding a lissencephaly type-1-like protein with WD40 repeats is required for plant height and male gametophyte formation in rice Planta 235 713 727 2012 22020753
  37. [45]Hiei Y Ohta S Komari T Kumashiro T Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA Plant J 6 271 282 1994 7920717
  38. [46]Bart R Chern M Park CJ Bartley L Ronald PC A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts Plant Methods 2 13 2006 16808845 PMC1524957
  39. [47]Kierzkowski D The Arabidopsis CBP20 targets the cap-binding complex to the nucleus, and is stabilized by CBP80 Plant J 59 814 825 2009 19453442
  40. [48]Kamachi K Yamaya T Mae T Ojima K A role for glutamine synthetase in the remobilization of leaf nitrogen during natural senescence in rice leaves Plant Physiol 96 411 417 1991 16668201 PMC1080785
  41. [50]Gamborg OL Miller RA Ojima K Nutrient requirements of suspension cultures of soybean root cells Exp Cell Res 50 151 158 1968 5650857

Cited by

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Authors
Jiang, L., Liu, X., Xiong, G., Liu, H., Chen, F., Wang, L., Meng, X., Liu, G., Yu, H., Yuan, Y., Yi, W., Zhao, L., Ma, H., He, Y., Wu, Z., Melcher, K., Qian, Q., Xu, H. E., Wang, Y., & Li, J.
Affiliations
  • Chinese Academy of Sciences (CN)
  • Van Andel Institute (US)
  • Chinese Academy of Agricultural Sciences (CN)
Corresponding author
  • Xu, H. Eric
  • Wang, Yonghong
  • Li, Jiayang
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2013-12-11
Volume
504
Issue
7480
Pages
401-405
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/nature12870
PMC
PMC5802366
OpenAlex
W1978058043
Cited by
868
References
3
Citations by year
2026: 52 · 2025: 75 · 2024: 89 · 2023: 72 · 2022: 90 · 2021: 62 · 2020: 84 · 2019: 71 · 2018: 55 · 2017: 62 · 2016: 51 · 2015: 56 · 2014: 47 · 2013: 1
FWCI
62.40
Type
journal article
Language
en
Update notices
erratum (2014-01-29)
Open access
Licence
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Funders
National Institute of General Medical Sciences (R01GM102545)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
strigolactone signalling, D3, SCF ubiquitin ligase, protein degradation, ubiquitination, corepressors, rice, shoot branching, GR24, hormone signaling, gene expression regulation
MeSH terms
Cloning, Molecular, Lactones, Models, Biological, Mutation, Plant Growth Regulators, Plant Proteins, Protein Binding, Oryza, Signal Transduction, Gene Expression Regulation, Plant, Ubiquitin, Multiprotein Complexes, Proteasome Endopeptidase Complex, Proteolysis

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