The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Systems-level metabolic flux profiling identifies fatty acid synthesis as a target for antiviral therapy
Open accessAbstract
Viruses rely on the metabolic network of their cellular hosts to provide energy and building blocks for viral replication. We developed a flux measurement approach based on liquid chromatography–tandem mass spectrometry to quantify changes in metabolic activity induced by human cytomegalovirus (HCMV). This approach reliably elucidated fluxes in cultured mammalian cells by monitoring metabolome labeling kinetics after feeding cells 13C-labeled forms of glucose and glutamine. Infection with HCMV markedly upregulated flux through much of the central carbon metabolism, including glycolysis. Particularly notable increases occurred in flux through the tricarboxylic acid cycle and its efflux to the fatty acid biosynthesis pathway. Pharmacological inhibition of fatty acid biosynthesis suppressed the replication of both HCMV and influenza A, another enveloped virus. These results show that fatty acid synthesis is essential for the replication of two divergent enveloped viruses and that systems-level metabolic flux profiling can identify metabolic targets for antiviral therapy.
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Cites
Identification of host proteins required for HIV infection through a functional genomic screen
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Identification of Proteins in Human Cytomegalovirus (HCMV) Particles: the HCMV Proteome
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Self-citationATP citrate lyase is an important component of cell growth and transformation
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Cancer-associated IDH1 mutations produce 2-hydroxyglutarate
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Self-citationThe Human Serum Metabolome (HUSERMET) Consortium, Dunn, W. B., Broadhurst, D., Begley, P., Zelena, E., Francis-McIntyre, S., Anderson, N., Brown, M., Knowles, J. D., Halsall, A., et al. (2011) · Nature Protocols · doi:10.1038/nprot.2011.335 · Cited by 3,294
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Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia
Metallo, C. M., Gameiro, P. A., Bell, E. L., Mattaini, K. R., Yang, J., Hiller, K., Jewell, C. M., Johnson, Z. R., Irvine, D. J., Guarente, L., et al. (2012) · Nature · doi:10.1038/nature10602 · Cited by 1,791
Quantitative flux analysis reveals folate-dependent NADPH production
Fan, J., Ye, J., Kamphorst, J. J., Shlomi, T., Thompson, C. B., & Rabinowitz, J. D. (2014) · Nature · doi:10.1038/nature13236 · Cited by 1,114
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Metadata
- Authors
- Munger, J., Bennett, B. D., Parikh, A., Feng, X.-J., McArdle, J., Rabitz, H. A., Shenk, T., & Rabinowitz, J. D.
- Venue
- Nature Biotechnology
- Published
- 2008-09-28
- Volume
- 26
- Issue
- 10
- Pages
- 1179-1186
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2126711569
- Cited by
- 635
- References
- 6
- FWCI
- 10.21
- Type
- journal article
- Language
- en
- Open access
- green
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- Munger, J., Bennett, B. D., Parikh, A., Feng, X.-J., McArdle, J., Rabitz, H. A., Shenk, T., & Rabinowitz, J. D. (2008). Systems-level metabolic flux profiling identifies fatty acid synthesis as a target for antiviral therapy. Nature Biotechnology. https://doi.org/10.1038/nbt.1500
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
