Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells
Abstract
Using a flow cytometry-based screen of commercial antibodies, we have identified cell-surface markers for the separation of pancreatic cell types derived from human embryonic stem (hES) cells. We show enrichment of pancreatic endoderm cells using CD142 and of endocrine cells using CD200 and CD318. After transplantation into mice, enriched pancreatic endoderm cells give rise to all the pancreatic lineages, including functional insulin-producing cells, demonstrating that they are pancreatic progenitors. In contrast, implanted, enriched polyhormonal endocrine cells principally give rise to glucagon cells. These antibodies will aid investigations that use pancreatic cells generated from pluripotent stem cells to study diabetes and pancreas biology.
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ViaCyte developed a stem cell-derived pancreatic endoderm cell population known as PEC-01, which they demonstrated to mature over several months in vivo into insulin-producing endocrine cells in rodent models.
“In particular, ViaCyte developed a stem cell-derived pancreatic endoderm cell population known as PEC-01, which they demonstrated to mature over several months in vivo into insulin-producing endocrine cells in rodent models.29–31”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [29]
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References 34 · Cited by 348
- Authors
- Kelly, O. G., Chan, M. Y., Martinson, L. A., Kadoya, K., Ostertag, T. M., Ross, K. G., Richardson, M., Carpenter, M. K., D'Amour, K. A., Kroon, E., Moorman, M., Baetge, E. E., & Bang, A. G.
- Affiliations
- ViaCyte (United States) (US)
- Corresponding author
- Kelly, Olivia G
- Venue
- Nature Biotechnology
- Published
- 2011-08
- Volume
- 29
- Issue
- 8
- Pages
- 750-756
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W1993222615
- Cited by
- 348
- References
- 34
- Citations by year
- 2026: 10 · 2025: 8 · 2024: 12 · 2023: 18 · 2022: 16 · 2021: 26 · 2020: 27 · 2019: 20 · 2018: 18 · 2017: 31 · 2016: 28 · 2015: 32 · 2014: 32 · 2013: 39 · 2012: 28
- FWCI
- 33.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- cell surface markers, human embryonic stem cells, pancreatic progenitors, tissue factor, CD200, insulin-producing cells, glucagon cells, pancreatic differentiation, transplantation, diabetes mellitus
- MeSH terms
- Animals, Antibodies, Cell Separation, Cells, Cultured, Endoderm, Flow Cytometry, Humans, Microscopy, Fluorescence, Pancreas, Transplantation, Heterologous, Biomarkers, Antigens, CD, Mice, SCID, Mice, Embryonic Stem Cells
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