The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with viral genetic barcoding
Open accessAbstract
Disentangling cellular heterogeneity is a challenge in many fields, particularly in the stem cell and cancer biology fields. Here we demonstrate how to combine viral genetic barcoding with high-throughput sequencing to track single cells in a heterogeneous population. We use this technique to track the in vivo differentiation of unitary hematopoietic stem cells (HSCs). The results are consistent with single-cell transplantation studies but require two orders of magnitude fewer mice. In addition to its high throughput, the high sensitivity of the technique allows for a direct examination of the clonality of sparse cell populations such as HSCs. We show how these capabilities offer a clonal perspective of the HSC differentiation process. In particular, our data suggest that HSCs do not equally contribute to blood cells after irradiation-mediated transplantation, and that two distinct HSC differentiation patterns co-exist in the same recipient mouse after irradiation. This technique can be applied to any virus-accessible cell type for both in vitro and in vivo processes.
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Cites
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Self-citationAssociation of reactive oxygen species levels and radioresistance in cancer stem cells
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Cited by
Regev, A., Teichmann, S. A., Lander, E. S., Amit, I., Benoist, C., Birney, E., Bodenmiller, B., Campbell, P., Carninci, P., Clatworthy, M., et al. (2017) · eLife · doi:10.7554/elife.27041 · Cited by 2,232
Self-citationSingle-Cell RNA-Seq with Waterfall Reveals Molecular Cascades underlying Adult Neurogenesis
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Lineage tracing on transcriptional landscapes links state to fate during differentiation
Weinreb, C., Rodriguez-Fraticelli, A., Camargo, F. D., & Klein, A. M. (2020) · Science · doi:10.1126/science.aaw3381 · Cited by 759
“We modified a classical strategy for clonal labeling by lentiviral delivery of inherited DNA barcodes (15, 16) to allow barcode detection using scSeq (17).”
MethodFundamental properties of unperturbed haematopoiesis from stem cells in vivo
Busch, K., Klapproth, K., Barile, M., Flossdorf, M., Holland-Letz, T., Schlenner, S. M., Reth, M., Höfer, T., & Rodewald, H.-R. (2015) · Nature · doi:10.1038/nature14242 · Cited by 769
Whole-organism lineage tracing by combinatorial and cumulative genome editing
McKenna, A., Findlay, G. M., Gagnon, J. A., Horwitz, M. S., Schier, A. F., & Shendure, J. (2016) · Science · doi:10.1126/science.aaf7907 · Cited by 766
Metadata
- Authors
- Lu, R., Neff, N. F., Quake, S. R., & Weissman, I. L.
- Venue
- Nature Biotechnology
- Published
- 2011-10
- Volume
- 29
- Issue
- 10
- Pages
- 928-933
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W1967350125
- Cited by
- 451
- References
- 50
- FWCI
- 9.52
- Type
- journal article
- Language
- en
- Open access
- green
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- Lu, R., Neff, N. F., Quake, S. R., & Weissman, I. L. (2011). Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with viral genetic barcoding. Nature Biotechnology. https://doi.org/10.1038/nbt.1977
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