The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Ribozyme-based insulator parts buffer synthetic circuits from genetic context
Open accessAbstract
Synthetic genetic programs are built from circuits that integrate sensors and implement temporal control of gene expression. Transcriptional circuits are layered by using promoters to carry the signal between circuits. In other words, the output promoter of one circuit serves as the input promoter to the next. Thus, connecting circuits requires physically connecting a promoter to the next circuit. We show that the sequence at the junction between the input promoter and circuit can affect the input-output response (transfer function) of the circuit. A library of putative sequences that might reduce (or buffer) such context effects, which we refer to as 'insulator parts', is screened in Escherichia coli. We find that ribozymes that cleave the 5′ untranslated region (5′-UTR) of the mRNA are effective insulators. They generate quantitatively identical transfer functions, irrespective of the identity of the input promoter. When these insulators are used to join synthetic gene circuits, the behavior of layered circuits can be predicted using a mathematical model. The inclusion of insulators will be critical in reliably permuting circuits to build different programs.
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Cites
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Ro, D.-K., Paradise, E. M., Ouellet, M., Fisher, K. J., Newman, K. L., Ndungu, J. M., Ho, K. A., Eachus, R. A., Ham, T. S., Kirby, J., et al. (2006) · Nature · doi:10.1038/nature04640 · Cited by 2,805
Foundations for engineering biology
Endy, D. (2005) · Nature · doi:10.1038/nature04342 · Cited by 1,511
Modular cell biology: retroactivity and insulation
Del Vecchio, D., Ninfa, A. J., & Sontag, E. D. (2008) · Molecular Systems Biology · doi:10.1038/msb4100204 · Cited by 519
Cited by
Genetic circuit design automation
Nielsen, A. A. K., Der, B. S., Shin, J., Vaidyanathan, P., Paralanov, V., Strychalski, E. A., Ross, D., Densmore, D., & Voigt, C. A. (2016) · Science · doi:10.1126/science.aac7341 · Cited by 1,020
Self-citationA brief history of synthetic biology
Cameron, D. E., Bashor, C. J., & Collins, J. J. (2014) · Nature Reviews Microbiology · doi:10.1038/nrmicro3239 · Cited by 933
Principles of genetic circuit design
Brophy, J. A. N., & Voigt, C. A. (2014) · Nature Methods · doi:10.1038/nmeth.2926 · Cited by 931
Self-citationGenomic mining of prokaryotic repressors for orthogonal logic gates
Stanton, B. C., Nielsen, A. A. K., Tamsir, A., Clancy, K., Peterson, T., & Voigt, C. A. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1411 · Cited by 379
Self-citationCourbet, A., Endy, D., Renard, E., Molina, F., & Bonnet, J. (2015) · Science Translational Medicine · doi:10.1126/scitranslmed.aaa3601 · Cited by 266
Metadata
- Authors
- Lou, C., Stanton, B., Chen, Y.-J., Munsky, B., & Voigt, C. A.
- Venue
- Nature Biotechnology
- Published
- 2012-10-03
- Volume
- 30
- Issue
- 11
- Pages
- 1137-1142
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W1596640364
- Cited by
- 397
- References
- 3
- FWCI
- 11.27
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Lou, C., Stanton, B., Chen, Y.-J., Munsky, B., & Voigt, C. A. (2012). Ribozyme-based insulator parts buffer synthetic circuits from genetic context. Nature Biotechnology. https://doi.org/10.1038/nbt.2401
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