The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies
Open accessAbstract
Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale precision on diffraction-limited instead of specialized super-resolution microscopes. ExM works by physically separating fluorescent probes after anchoring them to a swellable gel. The first ExM method did not result in the retention of native proteins in the gel and relied on custom-made reagents that are not widely available. Here we describe protein retention ExM (proExM), a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and streptavidin, and fluorescent proteins. We validated and demonstrated the utility of proExM for multicolor super-resolution (∼70 nm) imaging of cells and mammalian tissues on conventional microscopes.
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“0 13,14 , which marks neurons with random combinations of membrane anchored FPs.”
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A petavoxel fragment of human cerebral cortex reconstructed at nanoscale resolution
Shapson-Coe, A., Januszewski, M., Berger, D. R., Pope, A., Wu, Y., Blakely, T., Schalek, R. L., Li, P. H., Wang, S., Maitin-Shepard, J., et al. (2024) · Science · doi:10.1126/science.adk4858 · Cited by 361
Murakami, T. C., Mano, T., Saikawa, S., Horiguchi, S. A., Shigeta, D., Baba, K., Sekiya, H., Shimizu, Y., Tanaka, K. F., Kiyonari, H., et al. (2018) · Nature Neuroscience · doi:10.1038/s41593-018-0109-1 · Cited by 292
PLD3 affects axonal spheroids and network defects in Alzheimer’s disease
Yuan, P., Zhang, M., Tong, L., Morse, T. M., McDougal, R. A., Ding, H., Chan, D., Cai, Y., & Grutzendler, J. (2022) · Nature · doi:10.1038/s41586-022-05491-6 · Cited by 155
Metadata
- Authors
- Tillberg, P. W., Chen, F., Piatkevich, K. D., Zhao, Y., Yu, C.-C., English, B. P., Gao, L., Martorell, A., Suk, H.-J., Yoshida, F., DeGennaro, E. M., Roossien, D. H., Gong, G., Seneviratne, U., Tannenbaum, S. R., Desimone, R., Cai, D., & Boyden, E. S.
- Venue
- Nature Biotechnology
- Published
- 2016-07-04
- Volume
- 34
- Issue
- 9
- Pages
- 987-992
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2460281481
- Cited by
- 664
- References
- 3
- FWCI
- 25.97
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Tillberg, P. W., Chen, F., Piatkevich, K. D., Zhao, Y., Yu, C.-C., English, B. P., Gao, L., Martorell, A., Suk, H.-J., Yoshida, F., DeGennaro, E. M., Roossien, D. H., Gong, G., Seneviratne, U., Tannenbaum, S. R., Desimone, R., Cai, D., & Boyden, E. S. (2016). Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nature Biotechnology. https://doi.org/10.1038/nbt.3625
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