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Cited by 171Viral Infectious Diseases and Gene Expression in InsectsProtein purification and stabilityTransgenic Plants and Applications

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Culture pH Affects Expression Rates and Glycosylation of Recombinant Mouse Placental Lactogen Proteins by Chinese Hamster Ovary (CHO) Cells

Borys, Michael C., Linzer, Daniel I.H., Papoutsakis, Eleftherios T.·1993·Nature Biotechnology
DOI 10.1038/nbt0693-720
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Abstract

Glycosylation patterns and specific expression rates of the recombinant protein mouse placental lactogen-I (mPL-I) by Chinese hamster ovary (CHO) cells varied significantly over the extracellular pH (pHe) range of 6.1 to 8.7. The maximum specific mPL-I expression rates occurred between pHe 7.6 and 8.0. The pHe effect on protein expression was confirmed using a different CHO cell expressing the unglycosylated recombinant protein mouse placental lactogen-II (mPL-II). Decreases in the extent of glycosylation of mPL-I were observed at low (below 6.9) and high (above 8.2) pHe values. The pHe dependent variations in mPL-I accumulation in the supernatant as well as in glycosylation patterns were not the result of enzymatic degradation in the culture medium.

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Metadata

Authors
Borys, M. C., Linzer, D. I. H., & Papoutsakis, E. T.
Affiliations
Northwestern University (US)
ORCID
Papoutsakis, Eleftherios T.
Venue
Nature Biotechnology
Publisher
Springer Science and Business Media LLC
Published
1993-06
Volume
11
Issue
6
Pages
720-724
ISSN
  • 0733-222X (ISSN-L)
  • 1087-0156
  • 1546-1696
DOI
10.1038/nbt0693-720
OpenAlex
W1981449476
Cited by
171
References
42
Citations by year
2026: 1 · 2025: 3 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 4 · 2018: 6 · 2017: 7 · 2016: 4 · 2015: 9 · 2014: 7 · 2013: 10 · 2012: 6
FWCI
2.06
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (unspecified)
Locations
  • Nature Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01hd024518-11)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD24518)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
Keywords
extracellular pH, recombinant protein expression, glycosylation, CHO cells, protein secretion, culture medium pH
MeSH terms
Animals, Carbohydrate Sequence, Glycosylation, Cricetinae, Hydrogen-Ion Concentration, Molecular Sequence Data, Placental Lactogen, Recombinant Proteins, Immunoblotting, Gene Expression, CHO Cells, Culture Media, Conditioned, Mice

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