The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Amplification of the Zfy and Zfx Genes for Sex Identification in Humans, Cattle, Sheep and Goats
Abstract
We describe a quick and efficient method of determining the sex of DNA samples from humans, cattle, sheep, and goats. Using universal primers we have amplified 447 or 445 bp fragments from male or female genomic DNA corresponding to the ZFY or ZFX genes. Restriction fragment length polymorphism (RFLP) analysis of the fragments yielded specific banding patterns between the two sexes in these species. Horse, pig and rainbow trout fragments did not show RFLPs with the group of enzymes chosen for the other species. Use of this method would allow animal breeders to determine the sex of preimplantation embryos before transfer to recipient females. Human embryos at risk for sex-linked disorders could be sexed soon after in vitro fertilization (IVF) and options considered before implantation.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The male sex of our muskox specimen was denoted by the presence in our assembly of the X and Y chromosome-specific genes, ZFX (Acc KAL1286804) and ZFY (Acc KAL1286379)39, and the Y chromosome-specific gene, SRY (Acc KAL1286397).”
From Chromosomal-level reference genome assembly of muskox (Ovibos moschatus) from Banks Island in the Canadian Arctic, a resource for conservation genomics (2024) · cites this paper as [39]
Citations
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Detection of specific sequences among DNA fragments separated by gel electrophoresis
Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,245
Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification
Handyside, A. H., Kontogianni, E. H., Hardy, K., & Winston, R. M. L. (1990) · Nature · doi:10.1038/344768a0 · Cited by 1,601
(1967) · Annals of Internal Medicine · doi:10.7326/0003-4819-66-3-628_2 · Cited by 972
The sex-determining region of the human Y chromosome encodes a finger protein
Page, D. C., Mosher, R. A., Simpson, E. M., Fisher, E., Mardon, G., Pollack, J. R., McGillivray, B. C., de la Chapelle, A., & Brown, L. G. (1987) · Cell · doi:10.1016/0092-8674(87)90595-2 · Cited by 838
An Improved Method for Prenatal Diagnosis of Genetic Diseases by Analysis of Amplified DNA Sequences
Kogan, S. C., Doherty, M., & Gitschier, J. M. (1987) · New England Journal of Medicine · doi:10.1056/nejm198710153171603 · Cited by 798
Genetic evidence that ZFY is not the testis-determining factor
Palmer, M. S., Sinclair, A. H., Berta, P., Ellis, N. A., Goodfellow, P. N., Abbas, N., & Fellous, M. (1989) · Nature · doi:10.1038/342937a0 · Cited by 300
Schneider-Gädicke, A., Beer‐Romero, P., Brown, L. G., Nussbaum, R. L., & Page, D. C. (1989) · Cell · doi:10.1016/0092-8674(89)90061-5 · Cited by 287
The effects of storage of blood and isolated DNA on the integrity of DNA
Madisen, L., Hoar, D. I., Holroyd, C. D., Crisp, M., Hodes, M. E., & Reynolds, J. F. (1987) · American Journal of Medical Genetics · doi:10.1002/ajmg.1320270216 · Cited by 265
Zfy gene expression patterns are not compatible with a primary role in mouse sex determination
Koopman, P. A., Gubbay, J., Collignon, J., & Lovell-Badge, R. H. (1989) · Nature · doi:10.1038/342940a0 · Cited by 176
Research works printed but not linked: 2
- King W.A., 1984, Theriogenology, 5, 243
- Medrano J.F., 1990, Bio/Technology, 8, 144
Cited by
Sorted by citations
Items 301–323 of 323
ZES‐10: A new bovine MspI polymorphism detected by one primer
Osta, R., Rodellar, C., & Zaragoza, P. (1994) · Animal Genetics · doi:10.1111/j.1365-2052.1994.tb00220.x
UN MÉTODO PARA EL SEXAJE POR ADN DE ALPACA AMPLIFICANDO EL GEN SRY MEDIANTE PCR
Nino Arias, C., & Wilfredo Huanca, L. (2012) · Revista de Investigaciones Veterinarias del Perú · doi:10.15381/rivep.v20i2.607
Sex Identification of Bovine Meat Using Male Specific SRY and ZFY Genes
Shin, S.-C., Chung, K.-Y., & Chung, E.-R. (2007) · Korean Journal for Food Science of Animal Resources · doi:10.5851/kosfa.2007.27.3.351
Anoop, B. K., Yousuf, K. S. S. M., Sriram, M. P., Vaidya, N. G., Dinesh, C. K., & Vivekanandan, E. (2015) · Indian Journal of Fisheries
Sweny, J. M. (2006) · OakTrust (Texas A&M University Libraries)
A non-invasive approach examining North American river otter abundance and sociality
Brzeski, K. E. (2010) · California State University ScholarWorks (system-wide DSpace)
Sonoda, Y., Nakamura, M., MATSUE, M., Kubo, M., Ueno, Y., & Kurihara, M. (2014) · Journal of Japan Society of Civil Engineers Ser G (Environmental Research) · doi:10.2208/jscejer.70.ii_341
Goddard, K. (2009) · Wiley Encyclopedia of Forensic Science · doi:10.1002/9780470061589.fsa107
Goddard, K., & Espinoza, E. O. (2000) · Encyclopedia of Forensic Sciences · doi:10.1006/rwfs.2000.0618
Application of restriction endonuclease enzymes for sex determination in selected mammalian species
Özşensoy, Y. (2025) · Molecular Biology Reports · doi:10.1007/s11033-025-11149-z
Sex determination of Syrian hamster pups using placental tissuesPreprint
Kumpanenko, Y., Piessens, L., Neven, V., Dallmeier, K., & Alpizar, Y. A. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.11.22.689895
A Remarkable Specimen Record of an Apparently Wild Gray Wolf (Canis lupus) from Upstate New York
Kirchman, J. J., Feranec, R. S., Butera, J., & vonHoldt, B. M. (2025) · Northeastern Naturalist · doi:10.1656/045.032.0310
Shark sexing from forensic, archival, and developmental samples using sex-linked DNA markersPreprint
Akane, O., Kawaguchi, Y. W., Niwa, T., Uno, Y., & Kuraku, S. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.05.02.722412
Ryazanov, S. D., & Efimova, K. V. (2026) · Journal of Mammalian Evolution · doi:10.1007/s10914-026-09830-6
Đokić, M., Jojić, V., Lukić, P., Klisarić, N. B., Penezić, A., & Bugarski‐Stanojević, V. (2026) · Life · doi:10.3390/life16091408
Conservation genetics of enclosed black rhinoceros populations in Kenya
Cain, B. (2026) · e-space (Manchester Metropolitan University) · doi:10.83056/mmu.32434617
Identificação sexual da anta (Tapirus terrestris) por meio de marcadores de DNA
Pelizzon, C. (2010) · UNESP Institutional Repository (São Paulo State University)
Metadata
- Authors
- Aasen, E., & Medrano, J. F.
- Affiliations
- University of California, Davis (US)
- Venue
- Nature Biotechnology
- Published
- 1990-12
- Volume
- 8
- Issue
- 12
- Pages
- 1279-1281
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2022126715
- Cited by
- 323
- References
- 21
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 7 · 2023: 8 · 2022: 6 · 2021: 11 · 2020: 12 · 2019: 9 · 2018: 7 · 2017: 10 · 2016: 13 · 2015: 6 · 2014: 8 · 2013: 11 · 2012: 14
- FWCI
- 3.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Biotechnology(Faisalabad)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Prenatal Screening and Diagnostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Keywords
- sex determination, ZFY gene, sex-linked genes, restriction fragment length polymorphism, in vitro fertilization, sex-linked disorders, cattle, sheep, goats, human, universal primers
- MeSH terms
- Animals, Base Sequence, Cattle, DNA, DNA-Binding Proteins, Female, Goats, Humans, Male, Molecular Sequence Data, Oligonucleotides, Polymorphism, Restriction Fragment Length, Sex Determination Analysis, Sheep, Transcription Factors, Polymerase Chain Reaction, Kruppel-Like Transcription Factors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
