The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Ciliary entry of the kinesin-2 motor KIF17 is regulated by importin-β2 and RanGTP
Open accessAbstract
The biogenesis, maintenance and function of primary cilia are controlled through intraflagellar transport (IFT) driven by two kinesin-2 family members, the heterotrimeric KIF3A/KIF3B/KAP complex and the homodimeric KIF17 motor. How these motors and their cargoes gain access to the ciliary compartment is poorly understood. Here, we identify a ciliary localization signal (CLS) in the KIF17 tail domain that is necessary and sufficient for ciliary targeting. Similarities between the CLS and classic nuclear localization signals (NLSs) suggest that similar mechanisms regulate nuclear and ciliary import. We hypothesize that ciliary targeting of KIF17 is regulated by a ciliary-cytoplasmic gradient of the small GTPase Ran, with high levels of GTP-bound Ran (RanGTP) in the cilium. Consistent with this, cytoplasmic expression of GTP-locked Ran(G19V) disrupts the gradient and abolishes ciliary entry of KIF17. Furthermore, KIF17 interacts with the nuclear import protein importin-β2 in a manner dependent on the CLS and inhibited by RanGTP. We propose that Ran has a global role in regulating cellular compartmentalization by controlling the shuttling of cytoplasmic proteins into nuclear and ciliary compartments.
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Cites
Overview of Structure and Function of Mammalian Cilia
Satir, P., & Christensen, S. T. (2007) · Annual Review of Physiology · doi:10.1146/annurev.physiol.69.040705.141236 · Cited by 1,142 · cited 1×
“Deletion of the C-terminal tail domain abolished ciliary localization [KIF17(1-846), Fig.”
BackgroundBanaszynski, L. A., Chen, L.-C., Maynard-Smith, L. A., Ooi, A. G. L., & Wandless, T. J. (2006) · Cell · doi:10.1016/j.cell.2006.07.025 · Cited by 902
The proteome of the mouse photoreceptor sensory cilium complex
Liu, Q., Tan, G., Levenkova, N., Li, T., Pugh, E. N., Rux, J. J., Speicher, D. W., & Pierce, E. A. (2007) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m700054-mcp200 · Cited by 359
Cited by
Ciliogenesis: building the cell's antenna
Ishikawa, H., & Marshall, W. F. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3085 · Cited by 961
Open Sesame: How Transition Fibers and the Transition Zone Control Ciliary Composition
Garcia-Gonzalo, F. R., & Reiter, J. F. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a028134 · Cited by 290
Proteomic Analysis of Mammalian Primary Cilia
Ishikawa, H., Thompson, J., Yates, J. R., & Marshall, W. F. (2012) · Current Biology · doi:10.1016/j.cub.2012.01.031 · Cited by 275
Importazole, a Small Molecule Inhibitor of the Transport Receptor Importin-β
Soderholm, J. F., Bird, S. L., Kalab, P., Sampathkumar, Y., Hasegawa, K., Uehara-Bingen, M., Weis, K., & Heald, R. (2011) · ACS Chemical Biology · doi:10.1021/cb2000296 · Cited by 274
Stages of ciliogenesis and regulation of ciliary length
Avasthi, P., & Marshall, W. F. (2012) · Differentiation · doi:10.1016/j.diff.2011.11.015 · Cited by 234
Metadata
- Authors
- Dishinger, J. F., Kee, H. L., Jenkins, P. M., Fan, S., Hurd, T. W., Hammond, J. W., Truong, Y. N.-T., Margolis, B., Martens, J. R., & Verhey, K. J.
- Venue
- Nature Cell Biology
- Published
- 2010-06-06
- Volume
- 12
- Issue
- 7
- Pages
- 703-710
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2023843317
- Cited by
- 285
- References
- 3
- FWCI
- 18.27
- Type
- journal article
- Language
- en
- Open access
- green
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- Dishinger, J. F., Kee, H. L., Jenkins, P. M., Fan, S., Hurd, T. W., Hammond, J. W., Truong, Y. N.-T., Margolis, B., Martens, J. R., & Verhey, K. J. (2010). Ciliary entry of the kinesin-2 motor KIF17 is regulated by importin-β2 and RanGTP. Nature Cell Biology. https://doi.org/10.1038/ncb2073
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