Lineage specificity of primary cilia in the mouse embryo
Open accessAbstract
Primary cilia are required for vertebrate cells to respond to specific intercellular signals. Here we define when and where primary cilia appear in the mouse embryo using a transgenic line that expresses ARL13B-mCherry in cilia and Centrin 2-GFP in centrosomes. Primary cilia first appear on cells of the epiblast at E6.0 and are subsequently present on all derivatives of the epiblast. In contrast, extraembryonic cells of the visceral endoderm and trophectoderm lineages have centrosomes but no cilia. Stem cell lines derived from embryonic lineages recapitulate the in vivo pattern: epiblast stem cells are ciliated, whereas trophoblast stem cells and extraembryonic endoderm (XEN) stem cells lack cilia. Basal bodies in XEN cells are mature and can form cilia when the AURKA-HDAC6 cilium disassembly pathway is inhibited. The lineage-dependent distribution of cilia is stable throughout much of gestation, defining which cells in the placenta and yolk sac are able to respond to Hedgehog ligands.
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Quiescent cells can lose their cilium due to developmental programming or environmental insults such as mechanical stress.
“Quiescent cells can lose their cilium as a consequence of developmental programming 15–19 or in response to environmental insults such as mechanical stress 20.”
From Cellular signalling by primary cilia in development, organ function and disease (2019) · cites this paper as [18]
The Discussion (page unavailable) says cilia formation is controlled by stable lineage-based mechanisms and that visceral endoderm and XEN cells fail to form cilia. This supports developmental control of ciliation, but the paper does not establish that quiescent cells lose a cilium they previously had. The mechanical-stress clause has a separate citation and is not assessed against this source. (LLM, conf=0.84)
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References 51 · Cited by 174
- Authors
- Bangs, F. K., Schrode, N., Hadjantonakis, A.-K., & Anderson, K. V.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- Corresponding author
- Anderson, Kathryn V.
- Venue
- Nature Cell Biology
- Published
- 2015-01-19
- Volume
- 17
- Issue
- 2
- Pages
- 113-122
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- PMC
- PMC4406239
- OpenAlex
- W2020303151
- Cited by
- 174
- References
- 51
- Citations by year
- 2026: 13 · 2025: 16 · 2024: 11 · 2023: 17 · 2022: 19 · 2021: 17 · 2020: 19 · 2019: 12 · 2018: 15 · 2017: 7 · 2016: 17 · 2015: 11
- FWCI
- 11.46
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Memorial Sloan-Kettering Cancer Center
- New York State Stem Cell Science
- National Institutes of Health (4r37hd035455-16)
- National Cancer Institute (P30CA008748)
- National Institute of Neurological Disorders and Stroke (NS044385, R01NS044385)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD035455, R37HD035455, HD035455)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Hedgehog Signaling Pathway Studies
- Keywords
- primary cilia, ciliogenesis, mouse embryo, epiblast, extraembryonic lineages, visceral endoderm, trophectoderm, centrosome, basal bodies, ARL13B, centrin 2, AURKA, HDAC6, cilia disassembly, epiblast stem cells, trophoblast stem cells, Hedgehog signaling, placenta, yolk sac, lineage specificity
- MeSH terms
- Animals, Cell Line, Chickens, Cilia, Embryo, Mammalian, Endoderm, Female, Embryo Implantation, Pregnancy, Yolk Sac, Cell Lineage, ADP-Ribosylation Factors, Mice, Gastrulation, Basal Bodies
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