Lifelong haematopoiesis is established by hundreds of precursors throughout mammalian ontogeny
Open accessAbstract
Current dogma asserts that mammalian lifelong blood production is established by a small number of blood progenitors. However, this model is based on assays that require the disruption, transplantation and/or culture of embryonic tissues. Here, we used the sample-to-sample variance of a multicoloured lineage trace reporter to assess the frequency of emerging lifelong blood progenitors while avoiding the disruption, culture or transplantation of embryos. We find that approximately 719 Flk1+ mesodermal precursors, 633 VE-cadherin+ endothelial precursors and 545 Vav1+ nascent blood stem and progenitor cells emerge to establish the haematopoietic system at embryonic days (E)7-E8.5, E8.5-E11.5 and E11.5-E14.5, respectively. We also determined that the spatio-temporal recruitment of endothelial blood precursors begins at E8.5 and ends by E10.5, and that many c-Kit+ clusters of newly specified blood progenitors in the aorta are polyclonal in origin. Our work illuminates the dynamics of the developing mammalian blood system during homeostasis.
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The size of the HSC compartment changes dramatically during development and considerably increases in the transition into adulthood.
“The size of the HSC compartment also changes dramatically during development, and considerably increases in the transition into adulthood (Ganuza et al., 2017; Werner et al., 2015).”
From Unraveling Hematopoiesis through the Lens of Genomics (2020) · cites this paper as Ganuza et al., 2017
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References 56 · Cited by 94
- Authors
- Ganuza, M., Hall, T., Finkelstein, D., Chabot, A., Kang, G., & McKinney-Freeman, S.
- Affiliations
- St. Jude Children's Research Hospital (US)
- ORCID
- Venue
- Nature Cell Biology
- Published
- 2017-09-18
- Volume
- 19
- Issue
- 10
- Pages
- 1153-1163
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- PMC
- PMC5705075
- OpenAlex
- W2755845890
- Cited by
- 94
- References
- 56
- Citations by year
- 2026: 4 · 2025: 11 · 2024: 22 · 2023: 15 · 2022: 8 · 2021: 8 · 2020: 16 · 2019: 5 · 2018: 4
- FWCI
- 5.27
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Queen Mary Research Online (Queen Mary University of London) (submittedVersion)
- Funders
- National Cancer Institute (P30CA021765)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK104028, K01DK080846)
- Topics
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Keywords
- hematopoiesis, lineage tracing, polyclonal origin, dorsal aorta, mouse embryo
- MeSH terms
- Cadherin 5, Animals, Cell Differentiation, Cells, Cultured, Genotype, Gestational Age, Hematopoiesis, Hematopoietic Stem Cells, Luminescent Proteins, Mice, Inbred C57BL, Mice, Transgenic, Microscopy, Fluorescence, Models, Biological, Phenotype, Time Factors, Signal Transduction, Antigens, CD, Cadherins, Linear Models, Hematopoietic Stem Cell Transplantation, Gene Expression Regulation, Developmental, Coculture Techniques, Proto-Oncogene Proteins c-kit, Cell Lineage, Integrases, RNA, Untranslated, Vascular Endothelial Growth Factor Receptor-2, Endothelial Cells, Proto-Oncogene Proteins c-vav, Cell Tracking
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