Clonal reversal of ageing-associated stem cell lineage bias via a pluripotent intermediate
Open accessAbstract
Ageing associates with significant alterations in somatic/adult stem cells and therapies to counteract these might have profound benefits for health. In the blood, haematopoietic stem cell (HSC) ageing is linked to several functional shortcomings. However, besides the recent realization that individual HSCs might be preset differentially already from young age, HSCs might also age asynchronously. Evaluating the prospects for HSC rejuvenation therefore ultimately requires approaching those HSCs that are functionally affected by age. Here we combine genetic barcoding of aged murine HSCs with the generation of induced pluripotent stem (iPS) cells. This allows us to specifically focus on aged HSCs presenting with a pronounced lineage skewing, a hallmark of HSC ageing. Functional and molecular evaluations reveal haematopoiesis from these iPS clones to be indistinguishable from that associating with young mice. Our data thereby provide direct support to the notion that several key functional attributes of HSC ageing can be reversed.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Some aging phenotypes can be reversed by reprogramming aged HSCs to pluripotency and deriving definitive hematopoiesis from the resulting iPSCs.
“In fact, reversal of some aging phenotypes has been achieved by reprogramming old HSCs to pluripotency and subsequently generating definitive hematopoiesis from these induced pluripotent stem cells.107”
From Aging of hematopoietic stem cells (2018) · cites this paper as [107]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 29 · Cited by 59
- Authors
- Wahlestedt, M., Erlandsson, E., Kristiansen, T., Lu, R., Brakebusch, C., Weissman, I. L., Yuan, J., Martin-Gonzalez, J., & Bryder, D.
- Affiliations
- Lund University (SE)
- University of Southern California (US)
- University of Copenhagen (DK)
- Institute for Stem Cell Biology and Regenerative Medicine (US)
- Corresponding author
- Bryder, David
- Venue
- Nature Communications
- Published
- 2017-02-22
- Volume
- 8
- Issue
- 1
- Pages
- 14533-14533
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC5322498
- OpenAlex
- W2591403727
- Cited by
- 59
- References
- 29
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 4 · 2023: 5 · 2022: 7 · 2021: 10 · 2020: 8 · 2019: 5 · 2018: 2 · 2017: 4
- FWCI
- 3.99
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Lund University Publications (Lund University) (submittedVersion)
- Funders
- Cancerfonden (615068)
- Knut och Alice Wallenbergs Stiftelse
- Medical Research Council
- FP7 Ideas: European Research Council (615068)
- Topics
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- induced pluripotent stem cells, stem cell rejuvenation, clonal analysis, DNA barcoding, hematopoiesis
- MeSH terms
- Aging, Animals, Clone Cells, Hematopoietic Stem Cells, Mice, Inbred C57BL, T-Lymphocytes, Cellular Senescence, Gene Expression Regulation, Developmental, Cell Lineage, Induced Pluripotent Stem Cells, Cellular Reprogramming
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
