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Cited by 80Microtubule and mitosis dynamicsQuantum Dots Synthesis And PropertiesAdvanced Fluorescence Microscopy Techniques

Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots

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Katrukha, Eugene A., Mikhaylova, Marina, van Brakel, Hugo X., van Bergen en Henegouwen, Paul M., Akhmanova, Anna, Hoogenraad, Casper C., Kapitein, Lukas C.·2017·Nature Communications
DOI 10.1038/ncomms14772PMC PMC5364406

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Abstract

The cytoplasm is a highly complex and heterogeneous medium that is structured by the cytoskeleton. How local transport depends on the heterogeneous organization and dynamics of F-actin and microtubules is poorly understood. Here we use a novel delivery and functionalization strategy to utilize quantum dots (QDs) as probes for active and passive intracellular transport. Rapid imaging of non-functionalized QDs reveals two populations with a 100-fold difference in diffusion constant, with the faster fraction increasing upon actin depolymerization. When nanobody-functionalized QDs are targeted to different kinesin motor proteins, their trajectories do not display strong actin-induced transverse displacements, as suggested previously. Only kinesin-1 displays subtle directional fluctuations, because the subset of microtubules used by this motor undergoes prominent undulations. Using actin-targeting agents reveals that F-actin suppresses most microtubule shape remodelling, rather than promoting it. These results demonstrate how the spatial heterogeneity of the cytoskeleton imposes large variations in non-equilibrium intracellular dynamics.

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  • Stable microtubules are not static; they display sliding at approximately 0.7 μm/s, active curvature induction, deformation, breaking, and occasional depolymerization.

    “We found that, although these MTs were stable, they were far from static and displayed distinct behaviors such as sliding (at ∼0.7 µm/s, Fig. 3, C and F; Video 4), active curvature induction (Fig. 3 E; Video 5), deformation (Fig. S3 A), and breaking (Fig. S3 B), consistent with earlier reports (Xu et al., 2017; Robison et al., 2016; Katrukha et al., 2017).”

    From A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle (2023) · cites this paper as Katrukha et al., 2017

    In Results, under “Kinesin-1-targeted microtubules display undulations” (page unavailable), the paper reports that a subset of stable microtubules changed shape over time through undulation-like motion. The Discussion also describes active shape remodelling. Our copy does not report sliding at approximately 0.7 µm/s, breaking, or occasional microtubule depolymerization; its mention of depolymerization concerns actin. (LLM, conf=0.88)

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  • Stable microtubules have a curved appearance.

    “A characteristic feature of stable MTs is their curved appearance (Piperno et al., 1987; Friedman et al., 2010; Katrukha et al., 2017).”

    From A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle (2023) · cites this paper as Katrukha et al., 2017

    In Results, ‘Kinesin-1-targeted microtubules display undulations’ (Fig. 3e), the paper reports that the acetylated microtubules preferred by kinesin-1 are highly curved and describes them as a subset of stable microtubules. It does not establish curved appearance as a characteristic of stable microtubules generally. (LLM, conf=0.84)

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Cited by

Sorted by citations

Authors
Katrukha, E. A., Mikhaylova, M., van Brakel, H. X., van Bergen en Henegouwen, P. M., Akhmanova, A., Hoogenraad, C. C., & Kapitein, L. C.
Affiliations
Utrecht University (NL)
Corresponding author
Kapitein, Lukas C.
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2017-03-21
Volume
8
Issue
1
Pages
14772-14772
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/ncomms14772
PMC
PMC5364406
OpenAlex
W2952500106
Cited by
80
References
0
Citations by year
2026: 6 · 2025: 5 · 2024: 8 · 2023: 6 · 2022: 8 · 2021: 8 · 2020: 8 · 2019: 11 · 2018: 11 · 2017: 9
FWCI
5.56
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
  • Informationszentrum Lebenswissenschaften (ZB MED) (submittedVersion, cc-by)
Funders
  • Deutsche Forschungsgemeinschaft (Ml1923/1-1, 1923/1-1)
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek (865.10.010, NWO-ALW-VICI 865.10.010, 864.12.008, 864.12, 11421, NWO-ALW-VIDI 864.12.008)
  • Stichting voor de Technische Wetenschappen (11421, NWO-NANO 11421)
  • FP7 Ideas: European Research Council (336291)
  • FP7 People: Marie-Curie Actions (FP7-PEOPLE-2011-IEF)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Physical Sciences › Materials Science › Materials Chemistry › Quantum Dots Synthesis And Properties
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Keywords
cytoskeleton, intracellular transport, quantum dots, kinesin-1, microtubule dynamics, F-actin, actin depolymerization, diffusion heterogeneity, nonequilibrium dynamics
MeSH terms
Actins, Animals, Biological Transport, Biological Transport, Active, Chlorocebus aethiops, Cytoplasm, Cytoskeleton, Actin Cytoskeleton, Microtubules, Myosins, Kinesins, COS Cells, Quantum Dots

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