The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Identification and microbial production of a terpene-based advanced biofuel
Open accessAbstract
Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l−1. We produce bisabolene in Saccharomyces cerevisiae (>900 mg l−1), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into bisabolane. This work presents a framework for the identification of novel terpene-based advanced biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of biofuels. Advanced biofuels with comparable properties to petroleum-based fuels could be microbially produced from lignocellulosic biomass. In this study,Escherichia coliis engineered to produce bisabolene, the immediate precursor of bisabolane, a biosynthetic alternative to D2 diesel.
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Cites
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Ro, D.-K., Paradise, E. M., Ouellet, M., Fisher, K. J., Newman, K. L., Ndungu, J. M., Ho, K. A., Eachus, R. A., Ham, T. S., Kirby, J., et al. (2006) · Nature · doi:10.1038/nature04640 · Cited by 2,805
Self-citationEngineering Escherichia coli for production of functionalized terpenoids using plant P450s
Chang, M. C. Y., Eachus, R. A., Trieu, W., Ro, D.-K., & Keasling, J. D. (2007) · Nature Chemical Biology · doi:10.1038/nchembio875 · Cited by 364
Self-citationAnthony, J. R., Anthony, L. C., Nowroozi, F., Kwon, G., Newman, J. D., & Keasling, J. D. (2009) · Metabolic Engineering · doi:10.1016/j.ymben.2008.07.007 · Cited by 292
Self-citationRo, D.-K., Ouellet, M., Paradise, E. M., Burd, H., Eng, D., Paddon, C. J., Newman, J. D., & Keasling, J. D. (2008) · BMC Biotechnology · doi:10.1186/1472-6750-8-83 · Cited by 197
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Cited by
Principles of genetic circuit design
Brophy, J. A. N., & Voigt, C. A. (2014) · Nature Methods · doi:10.1038/nmeth.2926 · Cited by 931
Fuelling the future: microbial engineering for the production of sustainable biofuels
Liao, J. C., Mi, L., Pontrelli, S., & Luo, S. (2016) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.32 · Cited by 599
Engineering dynamic pathway regulation using stress-response promoters
Dahl, R. H., Zhang, F., Alonso-Gutierrez, J., Baidoo, E., Batth, T. S., Redding-Johanson, A. M., Petzold, C. J., Mukhopadhyay, A., Lee, T. S., Adams, P. D., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2689 · Cited by 473
Self-citationHong, K.-K., & Nielsen, J. (2012) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-0945-1 · Cited by 451
Davis, R., Tao, L., Tan, E., Biddy, M., Beckham, G., Scarlata, C., Jacobson, J., Cafferty, K., Ross, J., Lukas, J., et al. (2013) · doi:10.2172/1107470 · Cited by 437
Metadata
- Authors
- Peralta-Yahya, P. P., Ouellet, M., Chan, R., Mukhopadhyay, A., Keasling, J. D., & Lee, T. S.
- Venue
- Nature Communications
- Published
- 2011-09-27
- Volume
- 2
- Issue
- 1
- Pages
- 483-483
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2138885877
- Cited by
- 637
- References
- 4
- FWCI
- 14.81
- Type
- journal article
- Language
- en
- Open access
- hybrid
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