SNAP-25 regulates spine formation through postsynaptic binding to p140Cap
Open accessAbstract
Synaptosomal-associated protein of 25 kDa (SNAP-25) is a member of the Soluble N-ethylmaleimide-sensitive-factor attachment protein receptors (SNARE) protein family, required for exocytosis of synaptic vesicles and regulation of diverse ion channels. Here, we show that acute reduction of SNAP-25 expression leads to an immature phenotype of dendritic spines that are, consistently, less functional. Conversely, over-expression of SNAP-25 results in an increase in the density of mature, Postsynaptic Density protein 95 (PSD-95)-positive spines. The regulation of spine morphogenesis by SNAP-25 depends on the protein's ability to bind both the plasma membrane and the adaptor protein p140Cap, a key protein regulating actin cytoskeleton and spine formation. We propose that SNAP-25 allows the organization of the molecular apparatus needed for spine formation by recruiting and stabilizing p140Cap.
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SNAP-25 has recently been shown to facilitate the removal of kainate receptors (KARs) from the membrane surface.
“Moreover, SNAP-25 has recently been shown to facilitate the removal of kainate receptors (KARs), which are implicated in epileptogenesis, from the membrane surface (83, 90 -95).”
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References 60 · Cited by 71
Research works printed but not linked: 3
- Cheng, A. et al. Involvement of PGC-1alpha in the formation and maintenance of neuronal dendritic spines. Nat. Commun. 3, 1250 (2012).
- Ovsepian, S. V. & Dolly, J. O. Dendritic SNAREs add a new twist to the old neuron theory. Proc. Natl Acad. Sci. USA 108, 19113-19120 (2011).
- Meixner, A. et al. A QUICK screen for Lrrk2 interaction partners--leucine-rich repeat kinase 2 is involved in actin cytoskeleton dynamics. Mol. Cell Proteomics 10, 001172 (2011).
- Authors
- Tomasoni, R., Repetto, D., Morini, R., Elia, C., Gardoni, F., Di Luca, M., Turco, E., Defilippi, P., & Matteoli, M.
- Affiliations
- University of Milan (IT)
- University of Turin (IT)
- Corresponding author
- Matteoli, Michela
- ORCID
- Venue
- Nature Communications
- Published
- 2013-07-19
- Volume
- 4
- Issue
- 1
- Pages
- 2136-2136
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W1986504110
- Cited by
- 71
- References
- 60
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 4 · 2023: 8 · 2022: 6 · 2021: 1 · 2020: 7 · 2019: 6 · 2018: 9 · 2017: 5 · 2016: 5 · 2015: 7 · 2014: 5
- FWCI
- 2.29
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Archivio Istituzionale della Ricerca (Universita Degli Studi Di Milano) (submittedVersion, other-oa)
- Institutional Research Information System University of Turin (University of Turin) (submittedVersion)
- Funders
- European Commission (241498)
- Ministero della Salute (HEALTH-F2-2009-241498)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Keywords
- SNAP-25, dendritic spine formation, spine morphogenesis, postsynaptic density, PSD-95, SNARE proteins, synaptic vesicle exocytosis, actin cytoskeleton, synaptic plasticity
- MeSH terms
- Disks Large Homolog 4 Protein, Animals, Embryo, Mammalian, HeLa Cells, Hippocampus, Humans, Membrane Proteins, Actin Cytoskeleton, Protein Binding, Signal Transduction, Gene Expression Regulation, Developmental, Adaptor Proteins, Vesicular Transport, Intracellular Signaling Peptides and Proteins, Dendritic Spines, Synaptosomal-Associated Protein 25, Rats, Protein Stability, Primary Cell Culture
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