The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
A highly abundant bacteriophage discovered in the unknown sequences of human faecal metagenomes
Open accessAbstract
Metagenomics, or sequencing of the genetic material from a complete microbial community, is a promising tool to discover novel microbes and viruses. Viral metagenomes typically contain many unknown sequences. Here we describe the discovery of a previously unidentified bacteriophage present in the majority of published human faecal metagenomes, which we refer to as crAssphage. Its ~97 kbp genome is six times more abundant in publicly available metagenomes than all other known phages together; it comprises up to 90% and 22% of all reads in virus-like particle (VLP)-derived metagenomes and total community metagenomes, respectively; and it totals 1.68% of all human faecal metagenomic sequencing reads in the public databases. The majority of crAssphage-encoded proteins match no known sequences in the database, which is why it was not detected before. Using a new co-occurrence profiling approach, we predict a Bacteroides host for this phage, consistent with Bacteroides-related protein homologues and a unique carbohydrate-binding domain encoded in the phage genome. Metagenomic studies of microbial communities often report DNA sequences from unidentified viruses. Here, Dutilh et al. analyse metagenomic data to reveal the complete genome of an abundant, ubiquitous virus from human faeces, and predict that the virus infects bacteria of the Bacteroides group.
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Metagenomic compendium of 189,680 DNA viruses from the human gut microbiome
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Metadata
- Authors
- Dutilh, B. E., Cassman, N., McNair, K., Sanchez, S. E., Silva, G. G. Z., Boling, L., Barr, J. J., Speth, D. R., Seguritan, V., Aziz, R. K., Felts, B., Dinsdale, E. A., Mokili, J. L., & Edwards, R. A.
- Venue
- Nature Communications
- Published
- 2014-07-24
- Volume
- 5
- Issue
- 1
- Pages
- 4498-4498
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W1966912927
- Cited by
- 820
- References
- 15
- FWCI
- 35.24
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Dutilh, B. E., Cassman, N., McNair, K., Sanchez, S. E., Silva, G. G. Z., Boling, L., Barr, J. J., Speth, D. R., Seguritan, V., Aziz, R. K., Felts, B., Dinsdale, E. A., Mokili, J. L., & Edwards, R. A. (2014). A highly abundant bacteriophage discovered in the unknown sequences of human faecal metagenomes. Nature Communications. https://doi.org/10.1038/ncomms5498
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