The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
An optimized optogenetic clustering tool for probing protein interaction and function
Open accessAbstract
The Arabidopsis photoreceptor cryptochrome 2 (CRY2) was previously used as an optogenetic module, allowing spatiotemporal control of cellular processes with light. Here we report the development of a new CRY2-derived optogenetic module, ‘CRY2olig’, which induces rapid, robust, and reversible protein oligomerization in response to light. Using this module, we developed a novel protein interaction assay, Light-Induced Co-clustering, that can be used to interrogate protein interaction dynamics in live cells. In addition to use probing protein interactions, CRY2olig can also be used to induce and reversibly control diverse cellular processes with spatial and temporal resolution. Here we demonstrate disrupting clathrin-mediated endocytosis and promoting Arp2/3-mediated actin polymerization with light. These new CRY2-based approaches expand the growing arsenal of optogenetic strategies to probe cellular function. Protein–protein interactions are fundamental to nearly all molecular and cellular processes. Here Taslimi et al.describe a versatile new optogenetic module that can be used to visualize protein–protein interactions, as well as reversibly control them with light with spatiotemporal resolution.
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Cites
A novel genetic system to detect protein–protein interactions
Fields, S., & Song, O.-K. (1989) · Nature · doi:10.1038/340245a0 · Cited by 6,114
Millisecond-timescale, genetically targeted optical control of neural activity
Boyden, E. S., Zhang, F., Bamberg, E., Nagel, G., & Deisseroth, K. (2005) · Nature Neuroscience · doi:10.1038/nn1525 · Cited by 4,861
Phase transitions in the assembly of multivalent signalling proteins
Li, P., Banjade, S., Cheng, H.-C., Kim, S., Chen, B., Guo, L., Llaguno, M., Hollingsworth, J. V., King, D. S., Banani, S. F., et al. (2012) · Nature · doi:10.1038/nature10879 · Cited by 2,550
The development and application of optogenetics
Fenno, L., Yizhar, O., & Deisseroth, K. (2011) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-061010-113817 · Cited by 1,822
Eukaryotic Stress Granules: The Ins and Outs of Translation
Buchan, J. R., & Parker, R. (2009) · Molecular Cell · doi:10.1016/j.molcel.2009.11.020 · Cited by 1,492
Studying protein dynamics in living cells
Lippincott-Schwartz, J., Snapp, E., & Kenworthy, A. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35073068 · Cited by 1,190
Rapid blue-light–mediated induction of protein interactions in living cells
Kennedy, M. J., Hughes, R. M., Peteya, L. A., Schwartz, J. W., Ehlers, M. D., & Tucker, C. L. (2010) · Nature Methods · doi:10.1038/nmeth.1524 · Cited by 1,120
Self-citationStargazin regulates synaptic targeting of AMPA receptors by two distinct mechanisms
Chen, L., Chetkovich, D. M., Petralia, R. S., Sweeney, N. T., Kawasaki, Y., Wenthold, R. J., Bredt, D. S., & Nicoll, R. A. (2000) · Nature · doi:10.1038/35050030 · Cited by 1,111
AKAP signalling complexes: focal points in space and time
Wong, W., & Scott, J. D. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1527 · Cited by 1,073
Banaszynski, L. A., Chen, L.-C., Maynard-Smith, L. A., Ooi, A. G. L., & Wandless, T. J. (2006) · Cell · doi:10.1016/j.cell.2006.07.025 · Cited by 902
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Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets
Shin, Y., Berry, J., Pannucci, N., Haataja, M. P., Toettcher, J. E., & Brangwynne, C. P. (2017) · Cell · doi:10.1016/j.cell.2016.11.054 · Cited by 965
Physical principles of intracellular organization via active and passive phase transitions
Berry, J., Brangwynne, C. P., & Haataja, M. (2018) · Reports on Progress in Physics · doi:10.1088/1361-6633/aaa61e · Cited by 452
Protein Phase Separation Provides Long-Term Memory of Transient Spatial Stimuli
Dine, E., Gil, A. A., Uribe, G., Brangwynne, C. P., & Toettcher, J. E. (2018) · Cell Systems · doi:10.1016/j.cels.2018.05.002 · Cited by 182
LADL: light-activated dynamic looping for endogenous gene expression control
Kim, J. H., Rege, M., Valeri, J., Dunagin, M. C., Metzger, A., Titus, K. R., Gilgenast, T. G., Gong, W., Beagan, J. A., Raj, A., et al. (2019) · Nature Methods · doi:10.1038/s41592-019-0436-5 · Cited by 135
Billault-Chaumartin, I., Muriel, O., Michon, L., & Martin, S. G. (2022) · Current Biology · doi:10.1016/j.cub.2022.09.026 · Cited by 17
Metadata
- Authors
- Taslimi, A., Vrana, J. D., Chen, D., Borinskaya, S., Mayer, B. J., Kennedy, M. J., & Tucker, C. L.
- Venue
- Nature Communications
- Published
- 2014-09-18
- Volume
- 5
- Issue
- 1
- Pages
- 4925-4925
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2055045552
- Cited by
- 386
- References
- 44
- FWCI
- 9.66
- Type
- journal article
- Language
- en
- Open access
- bronze
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