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STED nanoscopy with fluorescent quantum dots
Open accessAbstract
The widely popular class of quantum-dot molecular labels could so far not be utilized as standard fluorescent probes in STED (stimulated emission depletion) nanoscopy. This is because broad quantum-dot excitation spectra extend deeply into the spectral bands used for STED, thus compromising the transient fluorescence silencing required for attaining super-resolution. Here we report the discovery that STED nanoscopy of several red-emitting commercially available quantum dots is in fact successfully realized by the increasingly popular 775 nm STED laser light. A resolution of presently ∼50 nm is demonstrated for single quantum dots, and sub-diffraction resolution is further shown for imaging of quantum-dot-labelled vimentin filaments in fibroblasts. The high quantum-dot photostability enables repeated STED recordings with >1,000 frames. In addition, we have evidence that the tendency of quantum-dot labels to blink is largely suppressed by combined action of excitation and STED beams. Quantum-dot STED significantly expands the realm of application of STED nanoscopy, and, given the high stability of these probes, holds promise for extended time-lapse imaging.
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Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
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Cited by
Application of semiconductor quantum dots in bioimaging and biosensing
Martynenko, I. V., Litvin, A. P., Purcell-Milton, F., Baranov, A. V., Fedorov, A. V., & Gun'ko, Y. K. (2017) · Journal of Materials Chemistry B · doi:10.1039/c7tb01425b · Cited by 382
Stimulated Emission Depletion Microscopy
Blom, H., & Widengren, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00653 · Cited by 339
Nanoparticles for super-resolution microscopy and single-molecule tracking
Jin, D., Xi, P., Wang, B., Zhang, L., Enderlein, J., & van Oijen, A. M. (2018) · Nature Methods · doi:10.1038/s41592-018-0012-4 · Cited by 283
Metadata
- Authors
- Hanne, J., Falk, H. J., Görlitz, F., Hoyer, P., Engelhardt, J., Sahl, S. J., & Hell, S. W.
- Venue
- Nature Communications
- Published
- 2015-05-18
- Volume
- 6
- Issue
- 1
- Pages
- 7127-7127
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W406599452
- Cited by
- 211
- References
- 1
- FWCI
- 12.70
- Type
- journal article
- Language
- en
- Open access
- gold
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- Hanne, J., Falk, H. J., Görlitz, F., Hoyer, P., Engelhardt, J., Sahl, S. J., & Hell, S. W. (2015). STED nanoscopy with fluorescent quantum dots. Nature Communications. https://doi.org/10.1038/ncomms8127
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