The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher
Open accessAbstract
Many cellular functions rely on DNA-binding proteins finding and associating to specific sites in the genome. Yet the mechanisms underlying the target search remain poorly understood, especially in the case of the highly organized mammalian cell nucleus. Using as a model Tet repressors (TetRs) searching for a multi-array locus, we quantitatively analyse the search process in human cells with single-molecule tracking and single-cell protein–DNA association measurements. We find that TetRs explore the nucleus and reach their target by 3D diffusion interspersed with transient interactions with non-cognate sites, consistent with the facilitated diffusion model. Remarkably, nonspecific binding times are broadly distributed, underlining a lack of clear delimitation between specific and nonspecific interactions. However, the search kinetics is not determined by diffusive transport but by the low association rate to nonspecific sites. Altogether, our results provide a comprehensive view of the recruitment dynamics of proteins at specific loci in mammalian cells.
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Cites
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A census of human transcription factors: function, expression and evolution
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From Silencing to Gene Expression
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Single-Molecule Dynamics of Enhanceosome Assembly in Embryonic Stem Cells
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Self-citationInsights from genomic profiling of transcription factors
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Hansen, A. S., Pustova, I., Cattoglio, C., Tjian, R., & Darzacq, X. (2017) · eLife · doi:10.7554/elife.25776 · Cited by 613
Self-citationMir, M., Stadler, M. R., Ortiz, S. A., Hannon, C. E., Harrison, M. M., Darzacq, X., & Eisen, M. B. (2018) · eLife · doi:10.7554/elife.40497 · Cited by 207
Self-citationDense Bicoid hubs accentuate binding along the morphogen gradient
Mir, M., Reimer, A., Haines, J. E., Li, X.-Y., Stadler, M., Garcia, H., Eisen, M. B., & Darzacq, X. (2017) · Genes & Development · doi:10.1101/gad.305078.117 · Cited by 192
Self-citationFunctional divergence of TBP homologs through distinct DNA-binding dynamics
Cui, J. H., Kwan, J. Z. J., Faghihi, A., Nguyen, T. F., & Teves, S. S. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf436 · Cited by 5
Metadata
- Authors
- Normanno, D., Boudarène, L., Dugast-Darzacq, C., Chen, J., Richter, C., Proux, F., Bénichou, O., Voituriez, R., Darzacq, X., & Dahan, M.
- Venue
- Nature Communications
- Published
- 2015-07-07
- Volume
- 6
- Issue
- 1
- Pages
- 7357-7357
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W793787745
- Cited by
- 194
- References
- 8
- FWCI
- 8.23
- Type
- journal article
- Language
- en
- Open access
- gold
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- Normanno, D., Boudarène, L., Dugast-Darzacq, C., Chen, J., Richter, C., Proux, F., Bénichou, O., Voituriez, R., Darzacq, X., & Dahan, M. (2015). Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher. Nature Communications. https://doi.org/10.1038/ncomms8357
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