Semaphorins guide the entry of dendritic cells into the lymphatics by activating myosin II
Open accessAbstract
The recirculation of leukocytes is essential for proper immune responses. However, the molecular mechanisms that regulate the entry of leukocytes into the lymphatics remain unclear. Here we show that plexin-A1, a principal receptor component for class III and class VI semaphorins, was crucially involved in the entry of dendritic cells (DCs) into the lymphatics. Additionally, we show that the semaphorin Sema3A, but not Sema6C or Sema6D, was required for DC transmigration and that Sema3A produced by the lymphatics promoted actomyosin contraction at the trailing edge of migrating DCs. Our findings not only demonstrate that semaphorin signals are involved in DC trafficking but also identify a previously unknown mechanism that induces actomyosin contraction as these cells pass through narrow gaps.
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Plexin-B1 has important roles in immune system activation and bone homeostasis in adult physiology.
“However Plexin-B1 has importance roles in immune system activation and bone homeostasis in adult physiology23–25”
From Regulation of cell distancing in peri-plaque glial nets by Plexin-B1 affects glial activation and amyloid compaction in Alzheimer’s disease (2024) · cites this paper as [24]
The Results section identifies plexin-A1, not plexin-B1, as important for dendritic-cell trafficking and antigen-specific T-cell responses. The References list a separate work on plexin-A1 and bone homeostasis. Searches for plexin-B1 and bone homeostasis found no passage addressing the claimed roles of plexin-B1; findings about A1 do not establish or rule out roles for B1. (LLM, conf=0.76)
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References 50 · Cited by 288
- Authors
- Takamatsu, H., Takegahara, N., Nakagawa, Y., Tomura, M., Taniguchi, M., Friedel, R. H., Rayburn, H., Tessier-Lavigne, M., Yoshida, Y., Okuno, T., Mizui, M., Kang, S., Nojima, S., Tsujimura, T., Nakatsuji, Y., Katayama, I., Toyofuku, T., Kikutani, H., & Kumanogoh, A.
- Affiliations
- Research Institute for Microbial Diseases, The University of Osaka (JP)
- Immunology Frontier Research Center, The University of Osaka (JP)
- RIKEN Center for Brain Science (JP)
- Sapporo Medical University (JP)
- Helmholtz Munich (DE)
- Stanford University (US)
- Cincinnati Children's Hospital Medical Center (US)
- Hyogo Medical University (JP)
- The University of Osaka (JP)
- Corresponding author
- Kumanogoh, Atsushi
- ORCID
- Venue
- Nature Immunology
- Published
- 2010-05-30
- Volume
- 11
- Issue
- 7
- Pages
- 594-600
- ISSN
- 1529-2908 (ISSN-L)
- 1529-2916
- PMC
- PMC3045806
- OpenAlex
- W2110859220
- Cited by
- 288
- References
- 50
- Citations by year
- 2026: 12 · 2025: 5 · 2024: 16 · 2023: 9 · 2022: 12 · 2021: 15 · 2020: 11 · 2019: 16 · 2018: 17 · 2017: 17 · 2016: 15 · 2015: 17 · 2014: 11 · 2013: 20 · 2012: 81
- FWCI
- 18.20
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Immunology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Site cant be reached (submittedVersion)
- Funders
- Japan Society for the Promotion of Science (21590196)
- National Institute of Neurological Disorders and Stroke (R01NS065048)
- Topics
- Health Sciences › Medicine › Oncology › Lymphatic System and Diseases
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Keywords
- semaphorin 3A, PLXNA1, dendritic cells, myosin II, actomyosin contraction, dendritic cell migration, lymph node drainage, leukocyte migration, semaphorin signaling, lymphatic vessels
- MeSH terms
- Actomyosin, Animals, Cell Movement, Cells, Cultured, Dendritic Cells, Immunity, Muscle Contraction, Nerve Tissue Proteins, Receptors, Cell Surface, Signal Transduction, Mice, Knockout, Adoptive Transfer, Myosin Type II, Neuropilin-1, Semaphorins, Lymphatic Vessels, Mice, Cell Migration Assays, Leukocyte, Gene Knock-In Techniques
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