RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes
Abstract
Lymphocyte function-associated antigen 1 (LFA-1) is relatively nonadhesive on resting lymphocytes; however, the mechanisms underlying changes in its adhesiveness are poorly understood. In this study, we generated a Jurkat T cell clone, J+hi1.14, that contained low amounts of mRNA for RhoH, a leukocyte-specific inhibitory Rho family member. J+hi1.14 cells expressed constitutively adhesive LFA-1 and the cells bound spontaneously to intracellular adhesion molecules 1, 2 and 3. Reconstitution of RhoH mRNA expression in J+hi1.14 cells reverted the adhesion phenotype to that of wild-type. We obtained similar results using RNA interference in peripheral blood lymphocytes. These data demonstrate that RhoH is required for maintenance of lymphocyte LFA-1 in a nonadhesive state.
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Overexpressing RhoH does not change LFA-1 mediated adhesion.
“However, when the authors tried to decrease LFA-1 mediated adhesion by overexpressing RhoH, no change in adhesion was detectable.”
From The small GTPase RhoH is an atypical regulator of haematopoietic cells (2008) · cites this paper as [63]
Decrease of RhoH expression activates LFA-1, rendering leukocytes adhesive and active.
“By searching for a deregulated LFA-1 phenotype using a retroviral insertion mutagenesis approach, Cherry et al. found that a decrease of RhoH expression activated LFA-1, thus rendering the leukocytes adhesive and therefore active.”
From The small GTPase RhoH is an atypical regulator of haematopoietic cells (2008) · cites this paper as [63]
RhoH is important for regulation of lymphocyte adhesion factor 1 (LFA-1).
“In a study aimed at identifying regulators of lymphocyte adhesion factor 1 (LFA-1), RhoH was found to be of importance [63].”
From The small GTPase RhoH is an atypical regulator of haematopoietic cells (2008) · cites this paper as [63]
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The small GTPase RhoH is an atypical regulator of haematopoietic cells
Fueller, F., & Kubatzky, K. F. (2008) · Cell Communication and Signaling · doi:10.1186/1478-811x-6-6 · Cited by 40 · see citing works
RhoH GTPase: A Key Regulator of Hematopoietic Cell Proliferation and Apoptosis?
Gu, Y., Zheng, Y., & Williams, D. A. (2005) · Cell Cycle · doi:10.4161/cc.4.2.1395 · Cited by 20 · see citing works
Zhou, J., Qian, M., Jiang, N., Wu, J., Feng, X., Yu, M., Min, Q., Xu, H., Yang, Y., Yang, Q., et al. (2024) · Journal of Clinical Immunology · doi:10.1007/s10875-024-01735-4 · Cited by 4 · see citing works
- Authors
- Cherry, L. K., Li, X., Schwab, P., Lim, B., & Klickstein, L. B.
- Affiliations
- Brigham and Women's Hospital (US)
- Beth Israel Deaconess Medical Center (US)
- Corresponding author
- Klickstein, Lloyd B
- Venue
- Nature Immunology
- Published
- 2004-08-08
- Volume
- 5
- Issue
- 9
- Pages
- 961-967
- ISSN
- 1529-2908 (ISSN-L)
- 1529-2916
- OpenAlex
- W2046477120
- Cited by
- 76
- References
- 0
- Citations by year
- 2024: 3 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 2 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 6 · 2012: 3
- FWCI
- 3.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK047636)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR047243, F32AR008632)
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- RhoH, LFA-1, integrin-mediated adhesion, lymphocytes, T cells, Rho GTPases, ICAM-1, ICAM-2, ICAM-3, lymphocyte adhesion, Jurkat cells, RNA interference
- MeSH terms
- Cell Adhesion, Electrophoresis, Polyacrylamide Gel, Flow Cytometry, Humans, Mutation, RNA, Messenger, T-Lymphocytes, Transcription Factors, Blotting, Southern, Signal Transduction, Lymphocyte Function-Associated Antigen-1, Intercellular Adhesion Molecule-1, Jurkat Cells, rho GTP-Binding Proteins
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