5′-triphosphate-siRNA: turning gene silencing and Rig-I activation against melanoma

Poeck, Hendrik; Besch, Robert; Maihoefer, Cornelius; Renn, Marcel; Tormo, Damia; Morskaya, Svetlana Shulga; Kirschnek, Susanne; Gaffal, Evelyn; Landsberg, Jennifer; Hellmuth, Johannes; Schmidt, Andreas; Anz, David; Bscheider, Michael; Schwerd, Tobias; Berking, Carola; Bourquin, Carole; Kalinke, Ulrich; Kremmer, Elisabeth; Kato, Hiroki; Akira, Shizuo; Meyers, Rachel; Häcker, Georg; Neuenhahn, Michael; Busch, Dirk; Ruland, Jürgen; Rothenfusser, Simon; Prinz, Marco; Hornung, Veit; Endres, Stefan; Tüting, Thomas; Hartmann, Gunther

doi:10.1038/nm.1887

5′-triphosphate-siRNA: turning gene silencing and Rig-I activation against melanoma

Genetic and epigenetic plasticity allows tumors to evade single-targeted treatments. Here we direct Bcl2-specific short interfering RNA (siRNA) with 5'-triphosphate ends (3p-siRNA) against melanoma. Recognition of 5'-triphosphate by the cytosolic antiviral helicase retinoic acid-induced protein I…