The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Human embryonic stem cells express an immunogenic nonhuman sialic acid
Open accessAbstract
Human embryonic stem cells (HESC) can potentially generate every body cell type, making them excellent candidates for cell- and tissue-replacement therapies. HESC are typically cultured with animal-derived 'serum replacements' on mouse feeder layers. Both of these are sources of the nonhuman sialic acid Neu5Gc, against which many humans have circulating antibodies. Both HESC and derived embryoid bodies metabolically incorporate substantial amounts of Neu5Gc under standard conditions. Exposure to human sera with antibodies specific for Neu5Gc resulted in binding of immunoglobulin and deposition of complement, which would lead to cell killing in vivo. Levels of Neu5Gc on HESC and embryoid bodies dropped after culture in heat-inactivated anti-Neu5Gc antibody–negative human serum, reducing binding of antibodies and complement from high-titer sera, while allowing maintenance of the undifferentiated state. Complete elimination of Neu5Gc would be likely to require using human serum with human feeder layers, ideally starting with fresh HESC that have never been exposed to animal products.
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Cites
Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo
Zufferey, R., Nagy, D., Mandel, R. J., Naldini, L., & Trono, D. (1997) · Nature Biotechnology · doi:10.1038/nbt0997-871 · Cited by 1,977
Cited by
Derivation of human embryonic stem cells in defined conditions
Ludwig, T. E., Levenstein, M. E., Jones, J. M., Berggren, W. T., Mitchen, E. R., Frane, J. L., Crandall, L. J., Daigh, C. A., Conard, K. R., Piekarczyk, M. S., et al. (2006) · Nature Biotechnology · doi:10.1038/nbt1177 · Cited by 1,114
Cardiac Stem Cells and Mechanisms of Myocardial Regeneration
Leri, A., Kajstura, J., & Anversa, P. (2005) · Physiological Reviews · doi:10.1152/physrev.00013.2005 · Cited by 451
The Potential of Adipose Stem Cells in Regenerative Medicine
Lindroos, B., Suuronen, R., & Miettinen, S. (2011) · Stem Cell Reviews and Reports · doi:10.1007/s12015-010-9193-7 · Cited by 441
Implications of the presence of N-glycolylneuraminic acid in recombinant therapeutic glycoproteins
Ghaderi, D., Taylor, R. E., Padler-Karavani, V., Diaz, S., & Varki, A. (2010) · Nature Biotechnology · doi:10.1038/nbt.1651 · Cited by 372
Self-citationGhaderi, D., Zhang, M., Hurtado-Ziola, N., & Varki, A. (2012) · Biotechnology and Genetic Engineering Reviews · doi:10.5661/bger-28-147 · Cited by 320
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Metadata
- Authors
- Martin, M. J., Muotri, A., Gage, F., & Varki, A.
- Venue
- Nature Medicine
- Published
- 2005-01-30
- Volume
- 11
- Issue
- 2
- Pages
- 228-232
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- OpenAlex
- W2120121992
- Cited by
- 932
- References
- 1
- FWCI
- 30.19
- Type
- journal article
- Language
- en
- Open access
- green
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