The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Detecting the conformation of individual proteins in live cells
Open accessAbstract
We combined single-molecule fluorescence resonance energy transfer (smFRET) with single-particle tracking in live cells to detect the in vivo conformation of individual proteins. We site-specifically labeled recombinant SNARE proteins with a FRET donor and acceptor before microinjecting them into cultured cells. Individual proteins rapidly incorporated into folded complexes at the cell membrane, demonstrating the potential of this method to reveal dynamic interactions within cells.
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Citations
Cites
Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution
Sutton, R. B., Fasshauer, D., Jahn, R., & Brunger, A. T. (1998) · Nature · doi:10.1038/26412 · Cited by 2,417
Cited by
Intrinsically disordered proteins: a 10-year recap
Tompa, P. (2012) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2012.08.004 · Cited by 631
Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
Hellenkamp, B., Schmid, S., Doroshenko, O., Opanasyuk, O., Kühnemuth, R., Rezaei Adariani, S., Ambrose, B., Aznauryan, M., Barth, A., Birkedal, V., et al. (2018) · Nature Methods · doi:10.1038/s41592-018-0085-0 · Cited by 519
Self-citationWei, G., Xi, W., Nussinov, R., & Ma, B. (2016) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00562 · Cited by 436
A toolkit and benchmark study for FRET-restrained high-precision structural modeling
Kalinin, S., Peulen, T., Sindbert, S., Rothwell, P. J., Berger, S., Restle, T., Goody, R. S., Gohlke, H., & Seidel, C. A. M. (2012) · Nature Methods · doi:10.1038/nmeth.2222 · Cited by 434
Measurement of single-cell dynamics
Spiller, D. G., Wood, C. D., Rand, D. A., & White, M. R. H. (2010) · Nature · doi:10.1038/nature09232 · Cited by 411
Metadata
- Authors
- Sakon, J. J., & Weninger, K. R.
- Venue
- Nature Methods
- Published
- 2010-01-31
- Volume
- 7
- Issue
- 3
- Pages
- 203-205
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2117028905
- Cited by
- 166
- References
- 1
- FWCI
- 8.56
- Type
- journal article
- Language
- en
- Open access
- green
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
