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Cited by 259

The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.

Proteomic and phosphoproteomic comparison of human ES and iPS cells

Open access
Phanstiel, Douglas H, Brumbaugh, Justin, Wenger, Craig D, Tian, Shulan, Probasco, Mitchell D, Bailey, Derek J, Swaney, Danielle L, Tervo, Mark A, Bolin, Jennifer M, Ruotti, Victor, Stewart, Ron, Thomson, James A, Coon, Joshua J·2011·Nature Methods

Abstract

Combining high-mass-accuracy mass spectrometry, isobaric tagging and software for multiplexed, large-scale protein quantification, we report deep proteomic coverage of four human embryonic stem cell and four induced pluripotent stem cell lines in biological triplicate. This 24-sample comparison resulted in a very large set of identified proteins and phosphorylation sites in pluripotent cells. The statistical analysis afforded by our approach revealed subtle but reproducible differences in protein expression and protein phosphorylation between embryonic stem cells and induced pluripotent cells. Merging these results with RNA-seq analysis data, we found functionally related differences across each tier of regulation. We also introduce the Stem Cell–Omics Repository (SCOR), a resource to collate and display quantitative information across multiple planes of measurement, including mRNA, protein and post-translational modifications.

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Metadata

Authors
Phanstiel, D. H., Brumbaugh, J., Wenger, C. D., Tian, S., Probasco, M. D., Bailey, D. J., Swaney, D. L., Tervo, M. A., Bolin, J. M., Ruotti, V., Stewart, R., Thomson, J. A., & Coon, J. J.
Venue
Nature Methods
Published
2011-09-11
Volume
8
Issue
10
Pages
821-827
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
OpenAlex
W2096217402
Cited by
259
References
6
FWCI
16.82
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Proteomic and phosphoproteomic comparison of human ES and iPS cells. https://app.cochat.ai/papers/10.1038/nmeth.1699
The paper
Phanstiel, D. H., Brumbaugh, J., Wenger, C. D., Tian, S., Probasco, M. D., Bailey, D. J., Swaney, D. L., Tervo, M. A., Bolin, J. M., Ruotti, V., Stewart, R., Thomson, J. A., & Coon, J. J. (2011). Proteomic and phosphoproteomic comparison of human ES and iPS cells. Nature Methods. https://doi.org/10.1038/nmeth.1699

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.