The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Genome-scale promoter engineering by coselection MAGE
Open accessAbstract
Multiplex automated genome engineering (MAGE) uses short oligonucleotides to scarlessly modify genomes; however, insertions >10 bases are still inefficient but can be improved substantially by selection of highly modified chromosomes. Here we describe 'coselection' MAGE (CoS-MAGE) to optimize biosynthesis of aromatic amino acid derivatives by combinatorially inserting multiple T7 promoters simultaneously into 12 genomic operons. Promoter libraries can be quickly generated to study gain-of-function epistatic interactions in gene networks.
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Cites
Programming cells by multiplex genome engineering and accelerated evolution
Wang, H. H., Isaacs, F. J., Carr, P. A., Sun, Z. Z., Xu, G., Forest, C. R., & Church, G. M. (2009) · Nature · doi:10.1038/nature08187 · Cited by 1,529 · cited 1×
“Using the MAGE-competent EcNR2 strain 2 , we first removed known feedback regulation and allosteric inhibition associated with Trp biosynthesis 7–10 , which involved nonsense inactivation of trpR , P148L mutation in aroF , M293T mutation in trpE , and D146N mutation in aroG .”
Self-citation
Cited by
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Jiang, W., Bikard, D., Cox, D., Zhang, F., & Marraffini, L. A. (2013) · Nature Biotechnology · doi:10.1038/nbt.2508 · Cited by 2,429
Genetic circuit design automation
Nielsen, A. A. K., Der, B. S., Shin, J., Vaidyanathan, P., Paralanov, V., Strychalski, E. A., Ross, D., Densmore, D., & Voigt, C. A. (2016) · Science · doi:10.1126/science.aac7341 · Cited by 1,020
Directed Evolution: Methodologies and Applications
Wang, Y., Xue, P., Cao, M., Yu, T., Lane, S. T., & Zhao, H. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00260 · Cited by 719
Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria
Farasat, I., Kushwaha, M., Collens, J., Easterbrook, M., Guido, M., & Salis, H. M. (2014) · Molecular Systems Biology · doi:10.15252/msb.20134955 · Cited by 235
Metadata
- Authors
- Wang, H. H., Kim, H., Cong, L., Jeong, J., Bang, D., & Church, G. M.
- Venue
- Nature Methods
- Published
- 2012-04-08
- Volume
- 9
- Issue
- 6
- Pages
- 591-593
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2082182783
- Cited by
- 230
- References
- 1
- FWCI
- 9.64
- Type
- journal article
- Language
- en
- Open access
- green
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- Wang, H. H., Kim, H., Cong, L., Jeong, J., Bang, D., & Church, G. M. (2012). Genome-scale promoter engineering by coselection MAGE. Nature Methods. https://doi.org/10.1038/nmeth.1971
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