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Faster STORM using compressed sensing
Open accessAbstract
In super-resolution microscopy methods based on single-molecule switching, the rate of accumulating single-molecule activation events often limits the time resolution. Here we developed a sparse-signal recovery technique using compressed sensing to analyze images with highly overlapping fluorescent spots. This method allows an activated fluorophore density an order of magnitude higher than what conventional single-molecule fitting methods can handle. Using this method, we demonstrated imaging microtubule dynamics in living cells with a time resolution of 3 s.
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Cited by
Spatially resolved, highly multiplexed RNA profiling in single cells
Chen, K. H., Boettiger, A. N., Moffitt, J. R., Wang, S., & Zhuang, X. (2015) · Science · doi:10.1126/science.aaa6090 · Cited by 2,969
Quantitative evaluation of software packages for single-molecule localization microscopy
Sage, D., Kirshner, H., Pengo, T., Stuurman, N., Min, J., Manley, S., & Unser, M. (2015) · Nature Methods · doi:10.1038/nmeth.3442 · Cited by 396
Compressed Sensing for Multidimensional Spectroscopy Experiments
Sanders, J. N., Saikin, S. K., Mostame, S., Andrade, X., Widom, J. R., Marcus, A. H., & Aspuru-Guzik, A. (2012) · The Journal of Physical Chemistry Letters · doi:10.1021/jz300988p · Cited by 83
Metadata
- Authors
- Zhu, L., Zhang, W., Elnatan, D., & Huang, B.
- Venue
- Nature Methods
- Published
- 2012-04-22
- Volume
- 9
- Issue
- 7
- Pages
- 721-723
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2043709234
- Cited by
- 518
- References
- 0
- FWCI
- 22.77
- Type
- journal article
- Language
- en
- Open access
- green
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