Whole-rat conditional gene knockout via genome editing
Abstract
Animal models with genetic modifications under temporal and/or spatial control are invaluable to functional genomics and medical research. Here we report the generation of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fertilized eggs. We generated rats with loxP-flanked (floxed) alleles and a tyrosine hydroxylase promoter-driven cre allele and demonstrated Cre-dependent gene disruption in vivo. Pronuclear microinjection of ZFNs, shown by our data to be an efficient and rapid method for creating conditional knockout rats, should also be applicable in other species.
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With advancements in rat genetic modification technologies, it is now possible to create rat models of senescent cell clearance.
“With advancements in genetic modification technologies in the rat [47–51], it is now possible to create rat models of senescent cell clearance.”
From Reducing Senescent Cell Burden in Aging and Disease (2020) · cites this paper as [48]
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References 16 · Cited by 77
- Authors
- Brown, A. J., Fisher, D. A., Kouranova, E., McCoy, A., Forbes, K., Wu, Y., Henry, R., Ji, D., Chambers, A., Warren, J., Shu, W., Weinstein, E. J., & Cui, X.
- Corresponding author
- Cui, Xiaoxia
- Venue
- Nature Methods
- Published
- 2013-06-09
- Volume
- 10
- Issue
- 7
- Pages
- 638-640
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2018164760
- Cited by
- 77
- References
- 16
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 5 · 2020: 5 · 2019: 6 · 2018: 4 · 2017: 8 · 2016: 5 · 2015: 10 · 2014: 10 · 2013: 10
- FWCI
- 5.22
- Type
- journal article
- Language
- en
- Update notices
- erratum (2013-09-27)
- Open access
- closed
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Keywords
- conditional knockout, zinc finger nucleases, loxP sites, Cre recombinase, floxed allele, pronuclear microinjection, tyrosine hydroxylase promoter, tissue-specific knockout, germline editing
- MeSH terms
- Animals, Deoxyribonucleases, Genetic Engineering, Transfection, Zinc Fingers, Genome, Rats, Rats, Transgenic, Gene Knockout Techniques
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