The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
The 3D OrbiSIMS—label-free metabolic imaging with subcellular lateral resolution and high mass-resolving power
Open accessAbstract
We report the development of a 3D OrbiSIMS instrument for label-free biomedical imaging. It combines the high spatial resolution of secondary ion mass spectrometry (SIMS; under 200 nm for inorganic species and under 2 μm for biomolecules) with the high mass-resolving power of an Orbitrap (>240,000 at m/z 200). This allows exogenous and endogenous metabolites to be visualized in 3D with subcellular resolution. We imaged the distribution of neurotransmitters—gamma-aminobutyric acid, dopamine and serotonin—with high spectroscopic confidence in the mouse hippocampus. We also putatively annotated and mapped the subcellular localization of 29 sulfoglycosphingolipids and 45 glycerophospholipids, and we confirmed lipid identities with tandem mass spectrometry. We demonstrated single-cell metabolomic profiling using rat alveolar macrophage cells incubated with different concentrations of the drug amiodarone, and we observed that the upregulation of phospholipid species and cholesterol is correlated with the accumulation of amiodarone.
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Cites
Stochastic Gene Expression in a Single Cell
Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002) · Science · doi:10.1126/science.1070919 · Cited by 5,441
Requirement of hippocampal neurogenesis for the behavioral effects of antidepressants
Santarelli, L., Saxe, M., Gross, C., Surget, A., Battaglia, F., Dulawa, S., Weisstaub, N., Lee, J., Duman, R., Arancio, O., et al. (2003) · Science · doi:10.1126/science.1083328 · Cited by 4,321
Diagnosing the decline in pharmaceutical R&D efficiency
Scannell, J. W., Blanckley, A., Boldon, H., & Warrington, B. (2012) · Nature Reviews Drug Discovery · doi:10.1038/nrd3681 · Cited by 1,981
(2006) · doi:10.1093/acprof:oso/9780195100273.001.0001 · Cited by 1,186
Michalski, A., Damoc, E., Hauschild, J.-P., Lange, O., Wieghaus, A., Makarov, A., Nagaraj, N., Cox, J., Mann, M., & Horning, S. (2011) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m111.011015 · Cited by 832
Self-citation
Cited by
Explainable multiview framework for dissecting spatial relationships from highly multiplexed data
(2022) · doi:10.1186/s13059-022-02663-5 · Cited by 297
Spatial Metabolomics and Imaging Mass Spectrometry in the Age of Artificial Intelligence
Alexandrov, T. (2020) · Annual Review of Biomedical Data Science · doi:10.1146/annurev-biodatasci-011420-031537 · Cited by 284
Porta Siegel, T., Hamm, G., Bunch, J., Cappell, J., Fletcher, J. S., & Schwamborn, K. (2018) · Molecular Imaging and Biology · doi:10.1007/s11307-018-1267-y · Cited by 187
Körber, A., Anthony, I. G. M., & Heeren, R. M. A. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.4c05249 · Cited by 88
Spatial pharmacology using mass spectrometry imaging
Rajbhandari, P., Neelakantan, T. V., Hosny, N., & Stockwell, B. R. (2024) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2023.11.003 · Cited by 49
Metadata
- Authors
- Passarelli, M. K., Pirkl, A., Moellers, R., Grinfeld, D., Kollmer, F., Havelund, R., Newman, C. F., Marshall, P. S., Arlinghaus, H., Alexander, M. R., West, A., Horning, S., Niehuis, E., Makarov, A., Dollery, C. T., & Gilmore, I. S.
- Venue
- Nature Methods
- Published
- 2017-11-13
- Volume
- 14
- Issue
- 12
- Pages
- 1175-1183
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2767546077
- Cited by
- 463
- References
- 5
- FWCI
- 19.32
- Type
- journal article
- Language
- en
- Open access
- green
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