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Cited by 361Neurobiology and Insect Physiology ResearchNeuroscience and Neuropharmacology ResearchOlfactory and Sensory Function Studies

Diversity and wiring variability of olfactory local interneurons in the Drosophila antennal lobe

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Chou, Ya-Hui, Spletter, Maria L, Yaksi, Emre, Leong, Jonathan C S, Wilson, Rachel I, Luo, Liqun·2010·Nature Neuroscience
DOI 10.1038/nn.2489PMC PMC2847188
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Abstract

Local interneurons are essential in information processing by neural circuits. Here we present a comprehensive genetic, anatomical and electrophysiological analysis of local interneurons (LNs) in the Drosophila melanogaster antennal lobe, the first olfactory processing center in the brain. We found LNs to be diverse in their neurotransmitter profiles, connectivity and physiological properties. Analysis of >1,500 individual LNs revealed principal morphological classes characterized by coarsely stereotyped glomerular innervation patterns. Some of these morphological classes showed distinct physiological properties. However, the finer-scale connectivity of an individual LN varied considerably across brains, and there was notable physiological variability within each morphological or genetic class. Finally, LN innervation required interaction with olfactory receptor neurons during development, and some individual variability also likely reflected LN-LN interactions. Our results reveal an unexpected degree of complexity and individual variation in an invertebrate neural circuit, a result that creates challenges for solving the Drosophila connectome.

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  • Antennal lobe local neurons are a morphologically variable and diverse class from two split transmitter hemilineages.

    “a difficult case concerning a morphologically variable and diverse class of neurons105 from two split transmitter hemilineages: local neurons of the antennal lobe”

    From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [105]

    The Results section, “Morphological diversity of LNs” (page unavailable), reports remarkable morphological diversity among antennal-lobe local neurons. The copy also describes two cell-body clusters and different neurotransmitter types, but it does not establish that these neurons come from two split transmitter hemilineages. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Half the local neurons previously categorized as LN2 'patchy' were predicted glutamatergic and half GABAergic.

    “Half the local neurons that had previously been categorized as LN2 ‘patchy’ local neurons,74,105 i.e., those that innervate discontinuous glomeruli in space, were predicted glutamatergic (cell type: lLN2P_a) and half GABAergic (cell types: lLN2P_b, lLN2P_c).”

    From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [105]

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Research works printed but not linked: 46

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Authors
Chou, Y.-H., Spletter, M. L., Yaksi, E., Leong, J. C. S., Wilson, R. I., & Luo, L.
Affiliations
  • Howard Hughes Medical Institute (US)
  • Harvard University (US)
Corresponding author
  • Wilson, Rachel I
  • Luo, Liqun
ORCID
Venue
Nature Neuroscience
Publisher
Springer Science and Business Media LLC
Published
2010-02-07
Volume
13
Issue
4
Pages
439-449
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
DOI
10.1038/nn.2489
PMC
PMC2847188
OpenAlex
W2127869243
Cited by
361
References
56
Citations by year
2026: 7 · 2025: 20 · 2024: 12 · 2023: 30 · 2022: 21 · 2021: 30 · 2020: 20 · 2019: 15 · 2018: 17 · 2017: 17 · 2016: 25 · 2015: 17 · 2014: 26 · 2013: 28 · 2012: 30
FWCI
13.33
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institute on Deafness and Other Communication Disorders (F31DC008277, R01DC008174, R01DC005982)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
Keywords
antennal lobe, Drosophila melanogaster, olfactory circuitry, neuronal heterogeneity, interindividual variability, neuronal connectivity, olfactory sensory neurons, neural development, connectome, electrophysiology
MeSH terms
Animals, Drosophila melanogaster, Interneurons, Nerve Net, Smell, Genetic Variation, Olfactory Receptor Neurons, Neurogenesis

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