Identification of distinct ChAT+ neurons and activity-dependent control of postnatal SVZ neurogenesis
Open accessAbstract
Postnatal and adult subventricular zone (SVZ) neurogenesis is believed to be primarily controlled by neural stem cell (NSC)-intrinsic mechanisms, interacting with extracellular and niche-driven cues. Although behavioral experiments and disease states have suggested possibilities for higher level inputs, it is unknown whether neural activity patterns from discrete circuits can directly regulate SVZ neurogenesis. We identified a previously unknown population of choline acetyltransferase (ChAT)(+) neurons residing in the rodent SVZ neurogenic niche. These neurons showed morphological and functional differences from neighboring striatal counterparts and released acetylcholine locally in an activity-dependent fashion. Optogenetic inhibition and stimulation of subependymal ChAT(+) neurons in vivo indicated that they were necessary and sufficient to control neurogenic proliferation. Furthermore, whole-cell recordings and biochemical experiments revealed direct SVZ NSC responses to local acetylcholine release, synergizing with fibroblast growth factor receptor activation to increase neuroblast production. These results reveal an unknown gateway connecting SVZ neurogenesis to neuronal activity-dependent control and suggest possibilities for modulating neuroregenerative capacities in health and disease.
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Neuron 3 may include ChAT+ local neurons that regulate V-SVZ cells.
“which may include ChAT+ local neurons that regulate V-SVZ cells (Paez-Gonzalez et al., 2014).”
From Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages (2019) · cites this paper as Paez-Gonzalez et al., 2014
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References 62 · Cited by 140
Research works printed but not linked: 1
- [31]Kordeli E Lambert S Bennett V AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier J Biol Chem 270 2352 2359 1995 7836469
- Authors
- Paez-Gonzalez, P., Asrican, B., Rodriguez, E., & Kuo, C. T.
- Affiliations
- Duke University (US)
- Corresponding author
- Kuo, Chay T
- Venue
- Nature Neuroscience
- Published
- 2014-06-01
- Volume
- 17
- Issue
- 7
- Pages
- 934-942
- ISSN
- 1097-6256 (ISSN-L)
- 1546-1726
- PMC
- PMC4122286
- OpenAlex
- W1968911616
- Cited by
- 140
- References
- 62
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 9 · 2023: 9 · 2022: 4 · 2021: 18 · 2020: 14 · 2019: 13 · 2018: 19 · 2017: 16 · 2016: 12 · 2015: 13 · 2014: 5
- FWCI
- 5.33
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Repositorio Institucional de la Universidad de Málaga (University of Málaga) (submittedVersion, other-oa)
- Funders
- National Institutes of Health (1dp2od004453-01)
- National Cancer Institute (P30CA014236)
- National Institute of General Medical Sciences (T32GM008441)
- National Institute of Neurological Disorders and Stroke (R01NS078192)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- cholinergic neurons, choline acetyltransferase, subventricular zone, postnatal neurogenesis, adult neurogenesis, neural stem cell proliferation, acetylcholine release, activity-dependent regulation, neural progenitor cells, fibroblast growth factor receptor, optogenetics, striatum
- MeSH terms
- Acetylcholine, Animals, Cells, Cultured, Cerebral Ventricles, Choline O-Acetyltransferase, Electrophoresis, Polyacrylamide Gel, Immunohistochemistry, Microscopy, Electron, Neurons, Blotting, Western, Receptors, Fibroblast Growth Factor, Patch-Clamp Techniques, Cell Proliferation, Mice, Neurogenesis, Electrophysiological Phenomena, Neural Stem Cells, Neuroimaging, Optogenetics
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