Calcium dynamics in astrocyte processes during neurovascular coupling
Open accessAbstract
Enhanced neuronal activity in the brain triggers a local increase in blood flow, termed functional hyperemia, via several mechanisms, including calcium (Ca(2+)) signaling in astrocytes. However, recent in vivo studies have questioned the role of astrocytes in functional hyperemia because of the slow and sparse dynamics of their somatic Ca(2+) signals and the absence of glutamate metabotropic receptor 5 in adults. Here, we reexamined their role in neurovascular coupling by selectively expressing a genetically encoded Ca(2+) sensor in astrocytes of the olfactory bulb. We show that in anesthetized mice, the physiological activation of olfactory sensory neuron (OSN) terminals reliably triggers Ca(2+) increases in astrocyte processes but not in somata. These Ca(2+) increases systematically precede the onset of functional hyperemia by 1-2 s, reestablishing astrocytes as potential regulators of neurovascular coupling.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cascade of metabolic and vascular events known as neurovascular coupling involves complex signaling among neurons, astrocytes, and the local vasculature and remains a topic of active research.
“Such a cascade of metabolic and vascular events, known as the neurovascular coupling, involves complex signaling among neurons, astrocytes, and the local vasculature (124, 126, 127), and remains a topic of active research enabled by emerging tools (128).”
From Electrophysiological source imaging: A noninvasive window to brain dynamics (2018) · cites this paper as [128]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 51 · Cited by 286
- Authors
- Otsu, Y., Couchman, K., Lyons, D. G., Collot, M., Agarwal, A., Mallet, J.-M., Pfrieger, F. W., Bergles, D. E., & Charpak, S.
- Affiliations
- Inserm (FR)
- Centre National de la Recherche Scientifique (FR)
- Johns Hopkins University (US)
- Corresponding author
- Charpak, Serge
- ORCID
- Venue
- Nature Neuroscience
- Published
- 2014-12-22
- Volume
- 18
- Issue
- 2
- Pages
- 210-218
- ISSN
- 1097-6256 (ISSN-L)
- 1546-1726
- PMC
- PMC4651918
- OpenAlex
- W2049584237
- Cited by
- 286
- References
- 51
- Citations by year
- 2026: 12 · 2025: 16 · 2024: 22 · 2023: 21 · 2022: 23 · 2021: 26 · 2020: 29 · 2019: 17 · 2018: 24 · 2017: 33 · 2016: 34 · 2015: 27 · 2012: 1
- FWCI
- 14.31
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- HAL (submittedVersion)
- Funders
- Institut National de la Santé et de la Recherche Médicale
- Fondation pour la Recherche Médicale
- National Institute of Mental Health (P50MH100024, P50MH084020, MH-084020)
- National Institute of Neurological Disorders and Stroke (P30NS050274)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- astrocytes, calcium signaling, neurovascular coupling, functional hyperemia, olfactory bulb, olfactory sensory neurons, astrocyte processes, genetically encoded calcium indicators, neuronal activity, blood flow regulation
- MeSH terms
- Animals, Astrocytes, Cerebrovascular Circulation, Mice, Transgenic, Olfactory Bulb, Synapses, Olfactory Receptor Neurons, Calcium Signaling, Mice, Receptor, Metabotropic Glutamate 5
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
