High resolution colonoscopy in live mice
Abstract
Endoscopy in humans is a powerful method for physicians to examine the gut for inflammatory or neoplastic changes. In medical and immunological research, animal models of intestinal diseases are established key tools to investigate the mucosal immune system, colitis and cancer development in the gut. Moreover, such models represent valid systems for testing of novel drugs. In the past, mice had to be killed in order to analyze colitis activity and tumor development. The following protocol describes a method to perform high resolution endoscopic monitoring of live mice. Mice developing colitis or colonic tumors are anesthetized and examined with a miniendoscope. The endoscope is introduced via the anus and the colon is carefully insufflated with an air pump. Endoscopic pictures obtained are of high quality and allow the monitoring and grading of tumors and inflammation. In addition, colonic biopsies can be taken. This protocol can be completed within 1 h.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Colitis severity was assessed using the established Murine Endoscopic Index of Colitis Severity (MEICS) scoring system based on five parameters.
“Murine Endoscopic Index of Colitis Severity [MEICS] scores for colitis severity were assigned at the end of the experiment on the basis of five parameters [translucency, granularity, fibrin, vascularity, and stool] according to an established scoring system 29–31.”
From PU.1-driven Th9 Cells Promote Colorectal Cancer in Experimental Colitis Models Through Il-6 Effects in Intestinal Epithelial Cells (2022) · cites this paper as [29]
The citation lists haven’t been fetched yet
References 15 · Cited by 369
- Authors
- Becker, C., Fantini, M. C., & Neurath, M. F.
- Affiliations
- Johannes Gutenberg University Mainz (DE)
- University of Rome Tor Vergata (IT)
- Corresponding author
- Neurath, M F
- Venue
- Nature Protocols
- Published
- 2006-12
- Volume
- 1
- Issue
- 6
- Pages
- 2900-2904
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2042149494
- Cited by
- 369
- References
- 15
- Citations by year
- 2026: 16 · 2025: 28 · 2024: 21 · 2023: 30 · 2022: 21 · 2021: 23 · 2020: 19 · 2019: 18 · 2018: 31 · 2017: 16 · 2016: 28 · 2015: 23 · 2014: 20 · 2013: 27 · 2012: 13
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Protocols (publishedVersion)
- PubMed (publishedVersion)
- UNICA IRIS Institutional Research Information System (University of Cagliari) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Health Sciences › Medicine › Oncology › Colorectal Cancer Screening and Detection
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal Bleeding Diagnosis and Treatment
- Keywords
- live mouse imaging, colitis, colon cancer, intestinal inflammation, tumor grading, colonic biopsy, mucosal immune system, drug testing
- MeSH terms
- Animals, Colon, Colonoscopy, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
