Paired-recordings from synaptically coupled cortical and hippocampal neurons in acute and cultured brain slices
Abstract
Analysis of synaptic transmission, synaptic plasticity, axonal processing, synaptic timing or electrical coupling requires the simultaneous recording of both the pre- and postsynaptic compartments. Paired-recording technique of monosynaptically connected neurons is also an appropriate technique to probe the function of small molecules (calcium buffers, peptides or small proteins) at presynaptic terminals that are too small to allow direct whole-cell patch-clamp recording. We describe here a protocol for obtaining, in acute and cultured slices, synaptically connected pairs of cortical and hippocampal neurons, with a reasonably high probability. The protocol includes four main stages (acute/cultured slice preparation, visualization, recording and analysis) and can be completed in approximately 4 h.
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Postsynaptic responses in AHA occurred with a latency of 5.0±0.4 ms, consistent with monosynaptic connectivity.
“Responses occurred with a latency of 5.0±0.4 ms, consistent with monosynaptic connectivity (Debanne et al., 2008).”
From Control of Stress-Induced Persistent Anxiety by an Extra-Amygdala Septohypothalamic Circuit (2014) · cites this paper as Debanne et al., 2008
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References 111 · Cited by 88
- Authors
- Debanne, D., Boudkkazi, S., Campanac, E., Cudmore, R. H., Giraud, P., Fronzaroli-Molinieres, L., Carlier, E., & Caillard, O.
- Affiliations
- Inserm (FR)
- Corresponding author
- Debanne, Dominique
- Venue
- Nature Protocols
- Published
- 2008-09-11
- Volume
- 3
- Issue
- 10
- Pages
- 1559-1568
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2086302525
- Cited by
- 88
- References
- 111
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 5 · 2020: 5 · 2019: 3 · 2018: 3 · 2017: 5 · 2016: 4 · 2015: 7 · 2014: 9 · 2013: 7 · 2012: 5
- FWCI
- 2.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Protocols (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-06-NEUR-0014)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- paired recordings, monosynaptic connections, cortical neurons, hippocampal neurons, synaptic transmission, synaptic plasticity, presynaptic terminals, calcium buffers, acute brain slices, electrical coupling
- MeSH terms
- Animals, Brain, Electrophysiology, Synaptic Transmission, Neurons, Tissue Culture Techniques, Mice, Rats, Synaptic Potentials
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