The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Site-specific N-terminal labeling of proteins using sortase-mediated reactions
Open accessAbstract
This protocol describes the use of sortase-mediated reactions to label the N terminus of any given protein of interest. The sortase recognition sequence, LPXTG (for Staphylococcus aureus sortase A) or LPXTA (for Streptococcus pyogenes sortase A), can be appended to a variety of probes such as fluorophores, biotin or even to other proteins. The protein to be labeled acts as a nucleophile by attacking the intermediate formed between the probe containing the LPXTG/A motif and the sortase enzyme. If sortase, the protein of interest and a suitably functionalized label are available, the reactions usually require less than 3 h.
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Cites
Webb, A. K. (2006) · Cited by 4,369
Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides
Kaiser, E., Colescott, R. L., Bossinger, C. D., & Cook, P. I. (1970) · Analytical Biochemistry · doi:10.1016/0003-2697(70)90146-6 · Cited by 3,516
Handbook of Proteolytic Enzymes
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Rowe, S. M., Miller, S., & Sorscher, E. J. (2005) · New England Journal of Medicine · doi:10.1056/nejmra043184 · Cited by 1,398
An Engineered Protein Tag for Multiprotein Labeling in Living Cells
Gautier, A., Juillerat, A., Heinis, C., Corrêa, I. R., Kindermann, M., Beaufils, F., & Johnsson, K. (2008) · Chemistry & Biology · doi:10.1016/j.chembiol.2008.01.007 · Cited by 1,087
Staphylococcus aureus Sortase, an Enzyme that Anchors Surface Proteins to the Cell Wall
Mazmanian, S. K., Liu, G., Ton-That, H., & Schneewind, O. (1999) · Science · doi:10.1126/science.285.5428.760 · Cited by 1,053
Choosing an effective protein bioconjugation strategy
Stephanopoulos, N., & Francis, M. B. (2011) · Nature Chemical Biology · doi:10.1038/nchembio.720 · Cited by 634
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The monitoring of reactions in solid-phase peptide synthesis with picric acid
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Unified Polymerization Mechanism for the Assembly of ASC-Dependent Inflammasomes
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The Current State of Peptide Drug Discovery: Back to the Future?
Henninot, A., Collins, J. C., & Nuss, J. M. (2018) · Journal of Medicinal Chemistry · doi:10.1021/acs.jmedchem.7b00318 · Cited by 1,113
Targeting the N terminus for site-selective protein modification
Rosen, C. B., & Francis, M. B. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2416 · Cited by 389
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Guimaraes, C. P., Witte, M. D., Theile, C. S., Bozkurt, G., Kundrat, L., Blom, A. E. M., & Ploegh, H. L. (2013) · Nature Protocols · doi:10.1038/nprot.2013.101 · Cited by 345
Self-citationMüller, D. J., Dumitru, A. C., Lo Giudice, C., Gaub, H. E., Hinterdorfer, P., Hummer, G., De Yoreo, J. J., Dufrêne, Y. F., & Alsteens, D. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00617 · Cited by 259
Metadata
- Authors
- Theile, C. S., Witte, M. D., Blom, A. E. M., Kundrat, L., Ploegh, H. L., & Guimaraes, C. P.
- Venue
- Nature Protocols
- Published
- 2013-08-29
- Volume
- 8
- Issue
- 9
- Pages
- 1800-1807
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2110776558
- Cited by
- 273
- References
- 40
- FWCI
- 6.59
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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