The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Organoid culture systems for prostate epithelial and cancer tissue
Open accessAbstract
This protocol describes a strategy for the generation of 3D prostate organoid cultures from healthy mouse and human prostate cells (either bulk or FACS-sorted single luminal and basal cells), metastatic prostate cancer lesions and circulating tumor cells. Organoids derived from healthy material contain the differentiated luminal and basal cell types, whereas organoids derived from prostate cancer tissue mimic the histology of the tumor. We explain how to establish these cultures in the fully defined serum-free conditioned medium that is required to sustain organoid growth. Starting with the plating of digested tissue material, full-grown organoids can usually be obtained in ∼2 weeks. The culture protocol we describe here is currently the only one that allows the growth of both the luminal and basal prostatic epithelial lineages, as well as the growth of advanced prostate cancers. Organoids established using this protocol can be used to study many different aspects of prostate biology, including homeostasis, tumorigenesis and drug discovery.
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Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
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Organoid Models and Applications in Biomedical Research
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Self-citationOrganoid Cultures Derived from Patients with Advanced Prostate Cancer
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Self-citationIn vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration
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Self-citationLgr5+ve Stem Cells Drive Self-Renewal in the Stomach and Build Long-Lived Gastric Units In Vitro
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Self-citationGrowing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications
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Progress and potential in organoid research
Rossi, G., Manfrin, A., & Lutolf, M. P. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0051-9 · Cited by 1,182
Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids
Hu, H., Gehart, H., Artegiani, B., LÖpez-Iglesias, C., Dekkers, F., Basak, O., van Es, J., Chuva de Sousa Lopes, S. M., Begthel, H., Korving, J., et al. (2018) · Cell · doi:10.1016/j.cell.2018.11.013 · Cited by 837
Self-citationDisease modeling in stem cell-derived 3D organoid systems
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Metadata
- Authors
- Drost, J., Karthaus, W. R., Gao, D., Driehuis, E., Sawyers, C. L., Chen, Y., & Clevers, H.
- Venue
- Nature Protocols
- Published
- 2016-01-21
- Volume
- 11
- Issue
- 2
- Pages
- 347-358
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2255555283
- Cited by
- 699
- References
- 26
- FWCI
- 38.66
- Type
- journal article
- Language
- en
- Open access
- green
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